In the present study, glucose oxidase was produced in shake culture by the parent Aspergillus niger with corn steep liquor as substrate. Glucose oxidase produced was then purified by subjecting to 60-85% saturation of ammonium sulfate precipitation and further purified by ion exchange and gel filtration chromatography respectively. Desalted sample of glucose oxidase showed the activity and spec...

Antigenic extracts of Streptococcus group E (SGE) were subjected to fractional ethanol precipitation, block (preparative) electrophoresis, and gel filtration for the purpose of separating the type antigen from the group antigen. Ethanol precipitation was ineffective in separating the substances. Block electrophoresis yielded serologically pure group antigen and a mixture of type and group antig...

Precipitation at pH 5.6 is found to improve the rabbit muscle troponin preparation. Further purification of troponin is achieved by treating troponin preparations with 0.1 M dithiothreitol, followed by the removal of contaminating tropomyosin by gel filtration. This is possible because through these purification procedures the molecular weight of tropomyosin as estimated by gel filtration remai...

Exo-alpha-N-acetylgalactosaminidase has been purified 8000-fold from Clostridium perfringens by gel filtration, ion exchange chromatography, isoelectric precipitation, and negative adsorption on human O type erythrocytes. The resulting enzyme is active at physiological pH and temperature. Phenyl glycosides, oligosaccharides, mucins, glycolipids, and cell membranes are substrates for this enzyme...

A highly sensitive enzyme-linked immunosorbent assay was used for the analysis of serum IgG reactivity against specific immunogenic Treponema pallidum proteins. Outer membrane treponemal proteins purified by preparative SDS-polyacrylamide gel electrophoresis were used as antigenic probes at concentrations as low as 100 ng per ml (5 ng per well in microtitre plates). Detection of anti-treponemal...

Two forms of proacrosin have been purified from ejacualted boar spermatozoa. The isolation method utilized benzamidine to inhibit the premeture activation of the zymogen and included pH precipitation, ammonium sulfate fractionation, and sodium chloride precipitation. Further purification was achieved by Sephadex G-200 FILTRATION OF THE PREPARATION AFTER IT WAS TREATED WITH 8 M urea. The overall...

INTRODUCTION Prolactin (PRL) exists in different forms in human serum. The predominant form is monomeric PRL (molecular mass 23 kDa) with smaller amounts of big PRL (molecular mass 50-60 kDa) and at times macroprolactin (molecular mass 150-170 kDa). Macroprolactin, generally considered to be biologically inactive, accounts for the major part of prolactin in some patients. Different immunoassays...

in this work, we report synthesis of surface modified superparamagnetic iron oxide nanoparticles (spion) by co-precipitation method using feso4.7h2o and fe2(so4)3.5h2o as precursors and trisodium citrate dihydrate as surfactant. surface modification of the as prepared samples was performed in pot by sol-gel precipitation method using tetraethoxysilane (teos). the structure, morphology, and magn...

BACKGROUND Many serological tests have been used for the diagnosis of human brucellosis. A new serological method is identified as Brucella Coombs gel test based on the principle of centrifugation gel system similar to the gel system used in blood group determination. In this system, if Brucella antibodies were present in the serum, antigen and antibody would remain as a pink complex on the gel...