Investigations have been made to develop an efficient protocol for identification and quantification of phenolic acids and flavonoids in hairy roots (HR) of Hypericum perforatum L. HR were induced from root segments of in vitro grown seedlings from H. perforatum, after co-cultivation with Agrobacterium rhizogenes A4. Transgenic status of HR was confirmed by PCR analysis using rolB specific prim...

Nonphotosynthetic cells of higher plants depend on the supply of a carbon substrate, usually sucrose, for cellular metabolism. Typically this sucrose is synthesized in leaves from the products of photosynthesis. Because of the lack of light below ground, roots do not generally photosynthesize (we note the exception of certain mangrove species and members of the Orchidaceae), although many roots...

biotechnology is providing us with a wide range of options for how we can use agricultural and commercial forestry lands. the cultivation of genetically modified (gm) crops on millions of hectares of lands and their injection into our food chain is a huge global genetic experiment involving all living beings. considering the fast pace of new advances in production of genetically modified crops,...

Transformed roots of the susceptible potato Solanum tuberosum L. cv. Desiree were inoculated with second-stage juveniles (J2) of Globodera rostochiensis pathotype Rol. Adult males emerged after 3-4 weeks and matings with females occurred. After 8 weeks gentle pressure on the eggs of maturing females released the J2 which were viable. Because this technique enables the production of vigorously g...

Nonphotosynthetic cells of higher plants depend on the supply of a carbon substrate, usually sucrose, for cellular metabolism. Typically this sucrose is synthesized in leaves from the products of photosynthesis. Because of the lack of light below ground, roots do not generally photosynthesize (we note the exception of certain mangrove species and members of the Orchidaceae), although many roots...

A direct corollary of the fundamental theorem of algebra is that p(x) can be factorized over the complex domain into a product an(x − r1)(x − r2) · · · (x − rn), where an is the leading coefficient and r1, r2, . . . , rn are all of its n complex roots. We will look at how to find roots, or zeros, of polynomials in one variable. The solution of multi-variate polynomials can often be transformed ...

Sections of hypocotyls, roots and leaves from Pentalinon andrieuxii plantlets were transiently transformed with Agrobacterium tumefaciens LBA4404 bearing the binary plasmid pCAMBIA2301 with an interrupted β-glucuronidase (GUS) gene. Histochemical GUS assays showed transient gene expression in all infected tissues, being older roots those which displayed the most intense GUS staining. To our kno...