..............................................................................................................................xviii Chapter 1: Introduction ...........................................................................................................1 1.1: Preface ..........................................................................................................................

A simple, rapid and efficient method for isolating genomic DNA from lychee (Litchi chinensis Sonn.) was developed. This modified CTAB protocol include the use of 2 M NaCl, PVP, 5% mercaptoethanol and 80% ethanol in the extraction as well as reducing the centrifugation times during the separation and precipitation of the DNA. Isolated genomic DNA showed high purity and high quantity following re...

Conventional genomic DNA extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. We developed a simple, time-saving and cost-efficient method for genomic DNA extraction from various types of organisms, using relatively innocuous reagents. The protocol employs a single purification ste...

Article Type Full Length Research Article Lactic acid bacteria are very significant to human health due to the production of some antimicrobial substances and ability to inhibit pathogenic bacteria. Furthermore, the bacteria are also used as starter culture in the production of various foods. The aim of this study was to evaluate five methods for the extraction and purification of DNA from 6 is...

We demonstrate the feasibility of using glass microbeads with a holographic barcode identifier to track DNA specimens in the molecular pathology laboratory. These beads can be added to peripheral blood specimens and are carried through automated DNA extraction protocols that use magnetic glass particles. We found that an adequate number of microbeads are consistently carried over during genomic...

Recovery, preservation and analysis of body fluid stains is an important aspect of forensic science. PCR-based typing of DNA extracted from recovered stains is often a crucial method to identify a perpetrator or exclude an innocent suspect. This paper reports an improved method of extracting genomic DNA from saliva stains deposited on human skin in simulated bite mark situations. Results of org...

The Genomic Resources Collection is a separate, independently managed part of the natural history collections Finnish Museum Natural History Luomus specifically intended for consumptive research. GRC policy deals with materials that are archived very purpose enabling study biological diversity at genome level, DNA extractions animal, fungal and plant specimens, animal tissue samples stored deep...

A number of methods have been described for the isolation of genomic DNA from whole blood. However, these described methods are either unreliable or too expensive to be used routinely in small diagnostic laboratories of developing countries. We have evaluated the slightly modified salt-out method for DNA isolation so that it can be applied on whole blood samples using a three step procedure: (1...