OBJECTIVE The aim of periodontal therapy is the healing of periodontal inflammation; the protection of the attachment and the alveolar bone; and the regeneration of the periodontal structures. In the therapy of periodontitis, supra- and subgingival scaling and root planing plays a main role. The procedure described combines perfect root cleaning without scaling and root planing and minimal inva...

A method was developed to obtain purified cryptosporidium oocysts from fecal samples. Oocysts were initially collected by centrifugation through a sucrose density gradient and further purified by passage through glass bead columns. The purified oocysts were antigenically active and sufficiently pure for immunological studies.

A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.

A monoclonal antibody to human antihemophilic factor (AHF, factor VIII) was derived from BALB/c mouse spleen cells fused with P3x63Ag8 mouse plasmacytoma cells. This antibody, harvested from culture medium or ascites fluid, reacted with purified AHF and with plasmas with normal subjects or classic hemophiliacs, as measured by enzyme-linked immunosorbent assay (ELISA), but not with plasmas from ...

PALMITOYLATION AND THE YEAST CASEIN KINASE YCK2byIRENE PAPANAYOTOUMay 2011Advisor: Dr. Nicholas G. DavisMajor: PharmacologyDegree: Doctor of Philosophy ! The Saccharomyces cerevisiae type I casein kinase Yck2 is tethered to thecytosolic face of the plasma membrane through palmitoylation of its two C-terminalcysteines. Yck2 palmitoylation is mediated by the Go...

Different methods for DNA isolation from Mycobacterium avium complex cultures were judged on the basis of their (1) ability to yield DNA from small culture samples, (2) speed of processing, (3) low number of manipulations and low potential for DNA crosscontamination, and (4) ability to provide DNA suitable for use as template in PCR and random amplified polymorphic DNA (RAPD) reactions. The gla...

We developed a new method to measure iron reduction at a distance based on depositing Fe(III) (hydr)oxide within nanoporous glass beads. In this "Fe-bead" system, Shewanella oneidensis reduces at least 86.5% of the iron in the absence of direct contact. Biofilm formation accompanies Fe-bead reduction and is observable both macro- and microscopically. Fe-bead reduction is catalyzed by live cells...

This double issue of Cybernetics and Human Knowing is dedicated to celebrating the life and work of Heinz von Foerster, cybernetician extraordinaire. He died on October 2nd, 2002. Mai von Foerster, his wife of over 60 years, died on June 22nd, 2003. They lived as a team and it is wrong to think of the work that appeared under the authorship of Heinz von Foerster without thinking of Mai's part i...

Materials and methods: A total of 68 molar bands were tried-in mouth of 17 patients. After try-in, 60 bands were cleaned in ultrasonic cleaning bath and then were equally divided into two groups (n = 30). Bands from group 1 were autoclaved in benchtop autoclave. Bands from group 2 were sterilized in glass bead sterilizer. After sterilization, the bands from both the groups were incubated in 50 ...

A bile acid disappearance test using an enzyme immunoassay for ursodeoxycholic acid (UDCA) is presented. The immunoassay employs an antiserum produced in rabbits with UDCA coupled by amide linkage to egg albumin. An antigen (UDCA)-enzyme (beta-D-galactosidase) complex was prepared by adding the N-hydroxy-succinimide ester of UDCA to beta-D-galactosidase in a molar ratio of 5000:1. The anti-UDCA...