The abundant biomass polysaccharides in nature can be degraded into oligo-oligosaccharides or monosaccharides. These sugars with small molecule used to produce ethanol, pharmaceutical products and other chemical materials. structure of plant is very complex, its degradation involves the synergistic catalysis various glycoside hydrolases, such as xylanase, glucanase, cellulose. Compared a single...

Enzymatic conversion of polysaccharides in the lignocellulosic biomass is currently subject intensive research and will be a key technology future biorefineries. Using bioinformatics approach, we previously identified putative endo-β-1,4-glucanase (DtCel5A) from Dictyoglomus thermophilum, chemoorganotrophic thermophilic bacterium. Here, structurally functionally characterize DtCel5A show that i...

The synthesis of 1,3-P-glucanase, P-N-acetylglucosaminidase and total protein was studied during autolysis of Neurospora crassa. Evidence for de novo protein synthesis during autolysis was obtained from the incorporation of [3H]tryptophan and [3H]phenylalanine into the protein pool and from the inhibitory effects of cycloheximide and puromycin. Glucose accumulated in the growth medium during au...


 The objective of the study was to isolate cellulase producing bacteria from natural sources and identify bacterial isolates that produce thermostable enzyme. Bacteria were isolated samples collected different locations like mango leaf litter, termite gut, composting yard (municipal solid waste, compost, soil), soil decaying paper wood, saline coastal belt by enrichment in broth containin...

In an investigation of the role of glucanases in modifying yeast cell walls at the location of new buds, vesicles derived from the endoplasmic reticulum, which are secreted locally into the cell wall of growing buds and may be involved in the secretion of glucanases, have been isolated.In yeast, exo-beta-1,3-glucanase (EC 3.2.1.6) is present both extra- and intracellularly. Exponentially growin...

Morphology changes in bacterial cellulose produced by Acetobacter xylinum ATCC23769 were ob­ served in the presence of /3 -glucodisaccharides such as gentiobiose and cellobiose. Endo-/3 -1,4-glucanase ac­ tivity in culture broth was higher than that in the absence of those sugars. So we have investigated the prop­ erties of endo-/3 -1,4-glucanase (AEG) produced by this bacterium. This enzyme co...

purification of extra-cellular cellulolytic enzymes from c e l l u l o mssp. strain 0, isolated from forest soil in the north of iran , was studied by using gel filtration and ion-exchange chromatography. two endo-glucanases (endo-i & endo-ii) and one exo-glucanase (exo-i) were purified to hornogenity . the purified enzymes had molecular weights of about 39.000,70.000 and 90.000 daltons for end...

endo-1,4-β-Glucanase (endo-cellulase, EC 3.2.1.4) is one of the most widely used enzymes in industry. Despite its importance, improved methods for the rapid, selective, quantitative assay of this enzyme have been slow to emerge. In 2014, a novel enzyme-coupled assay that addressed many of the limitations of the existing assay methodology was reported. This involved the use of a bifunctional sub...

A culture filtrate of Bacillus circulans KA-304 grown on a cell-wall preparation of Schizophyllum commune has an activity to form protoplasts from S. commune mycelia. alpha-1,3-Glucanase and chitinase I, which were isolated from the filtrate, did not form the protoplast by itself while a mixture of them showed protoplast-forming activity. Streptomyces cyaneus SP-27 was isolated based on the pro...

The results presented demonstrate that microbial pathogens of plants have the ability to secrete proteins which effectively inhibit an enzyme synthesized by the host; an enzyme whose substrate is a constituent of the cell wall of the pathogen. The system in which this was discovered is the anthracnose-causing fungal pathogen (Colletotrichum lindemuthianum) and its host, the French bean (Phaseol...