The glucose isomerase of Streptomyces albus has been crystallised from a dilute solution of magnesium chloride buffered at a pH of 6.8-7.0. The crystals are in the space group I222 with cell dimensions a = 93.9 A, b = 99.5 A and c = 102.9 A. There is one monomer of the tetrameric molecule per asymmetric unit of the crystal and the packing density is 2.93 A3.Da-1. The tetramer sits on the 222 sy...

A fructose diphosphatase-phosphofructokinase substrate cycle has been reconstructed in vitro to provide a system that recycles fructose 6-phosphate and hydrolyses ATP to ADP and P(i). The concerted actions of glucose phosphate isomerase, phosphofructokinase, aldolase and triose phosphate isomerase catalysed the loss of (3)H from [5-(3)H,U-(14)C]glucose 6-phosphate. This was used as the basis of...

The sulfosugar sulfoquinovose (SQ) is produced by essentially all photosynthetic organisms on Earth and metabolized bacteria through the process of sulfoglycolysis. sulfoglycolytic Embden–Meyerhof–Parnas pathway metabolizes SQ to produce dihydroxyacetone phosphate sulfolactaldehyde analogous classical glycolysis for metabolism glucose-6-phosphate, though former only provides one C3 fragment cen...

Abstract Crop production and growth are severely affected by salt stress. Nevertheless, the bio-fertilizer vermicompost (VC) can be participated as a potent inhibitor of salinity on plant crop regulating photosynthetic efficiency. We investigated effect VC performance salt-stressed broad bean ( Vicia faba L. Aspani cultivar). Seeds were grown in soil mixture; clay sand ratio 1:2 volume with fiv...

NADPH formation during growth on glucose was studied in wild type Escherichia coli and in mutants affected, directly or indirectly, in the NADPH-forming reactions glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase, and energy-linked transhydrogenase. The main technique was to grow cells in media containing glucose labeled uniformly with ‘“C and also containing glu...

It is widely accepted that many phase transitions do not follow nucleation pathways as envisaged by the classical nucleation theory. Many substances can traverse intermediate states before arriving at the stable phase. The apparent ubiquity of multi-step nucleation has made the inverse question relevant: does multistep nucleation always dominate single-step pathways? Here we provide an explicit...

Dehydration may change the crystal lattice and affect the mosaicity, resolution and quality of X-ray diffraction data. A dehydrating environment can be generated around a crystal in several ways with various degrees of precision and complexity. This study uses a high-precision crystal humidifier/dehumidifier to provide an airstream of known relative humidity in which the crystals are mounted: a...

The glycolytic enzyme phosphoglucose isomerase catalyses the reversible isomerization of glucose 6-phosphate to fructose 6-phosphate. The phosphoglucose isomerase from the hyperthermophilic archaeon Pyrococcus furiosus, which shows no sequence similarity to any known bacterial or eukaryotic phosphoglucose isomerase, has been cloned and overexpressed in Escherichia coli, purified and subsequentl...

Determinations of the salt sensitivity of enzymes extracted from the halophilic alga Dunaliella viridis revealed that pentose phosphate isomerase, ribulose diphosphate carboxylase, glucose-6-phosphate dehydrogenase, and phosphohexose isomerase were inhibited by NaCl concentrations far lower than that in the growth medium (3.75 m). The inhibition was reversible and was not prevented by preparing...