The effects of ionic liquids (ILs) on the catalytic activity of enzymes were studied by approaches of electrochemistry and quantum chemistry calculation in this work. Three types of ILs, namely, [bmpyri]BF(4), [bmpyrro]BF(4), and [bmim]BF(4), were selected to address the effects of different types of ILs on the electrocatalytic activity of glucose oxidase (GOx) toward the oxidation of glucose. ...

Protein conformation associates with particular properties of proteins and relates to protein-mediated diseases. Detailed elucidation of secondary and tertiary formation, stability, and the structural and dynamic properties of proteins has been one of the main topics studied in chemistry and biology. In this work, the conformation changes in glucose oxidase (GOx) induced by the graphene oxide (...

We have recently reported on dodecamer peptides (HPPMDFHKAMTR, CHPQPLKSRNPL) which recognize 52-58th and 197-203rd amino acid sequences of glucose oxidase (GOx) by screening via a phage random peptide library. In this study, a side-chain protected peptide monomer (PPM) was synthesized using two peptides (HPPMDFHKAMTR, SHPQPLKSRNPL) and acryloyl chloride. The peptide-conjugated polymer (PCP) was...

The reconstitution of apo-glucose oxidase (apo-GOx) on single-walled carbon nanotubes (SWNTs) functionalized with the cofactor, flavin adenine dinucleotide (FAD), greatly improved electron transfer turnover rate of the redox reactions in glucose sensing with glucose sensors. The research reported here is aimed to better understand molecular details of affection of the charging SWNT to the confo...

In order to improve its stability and catalytic rate in flour, the immobilization of glucose oxidase (GOX) was investigated in this work. The enzyme was encapsulated in calcium alginate-chitosan microspheres (CACM) using an emulsification-internal gelation-GOX adsorption-chitosan coating method. The interaction between alginate and chitosan was confirmed by infrared spectroscopy (IR). The resul...

Synergistic strategies by combining nanoreactors and prodrugs hold tremendous potential in anticancer treatment. However, precise death of target cancer cells remains a significant challenge due to the absence an elaborate targeting strategy. Here, dual-targeting approach that combines action H2O2-producing folate receptor-targeted with cyclooxygenase-2 (COX-2) targeted prodrug is reported. A f...

BACKGROUND Minimally invasive glucose biosensors with increased functional longevity form one of the most promising techniques for continuous glucose monitoring. In the present study, we developed a novel nanoengineered microsphere formulation comprising alginate microsphere glucose sensors and anti-inflammatory-drug-loaded alginate microspheres. METHODS The formulation was prepared and chara...

A novel amperometric sensor that integrates two independent measurement schemes into a single chip for detection of glucose is fabricated. The sensor uses micro-patterned Prussian blue (PB) and ferrocene modified glucose oxidase covered by a thin Nafion membrane. We have developed an amperometric sensor for the detection of glucose that integrates two measurement schemes into a single chip. For...

Compartmentalization is a ubiquitous feature of life, with membrane interface that regulates molecular transport and exhibits bioactivities. An artificial enzyme-integrated cascade system can mimic such interactive properties across different length scales for in vitro application. Here, it shown reticulated nanoporous framework nano-caged enzymes presents the first modular macroscale platform ...

Enzyme entrapped in an electrosynthesized polymer film on a high-surface-area electrode is shown to be an attractive platform for the important class of amperometric biosensors based on oxidases and electrooxidation of the H2O2 generated by enzyme-catalyzed oxidation of analyte. Two Pt electrode surface morphologies, Pt black (Pt-BLK) and Pt nanowire brush (Pt-NW) were electrodeposited from chl...