The catalase within normal, intact human erythrocytes was completely inactivated with amino triazole. The rate of 14CO2 evolution, when the cells were subsequently incubated with 14C-labeled glucose, provided a measure of the rate at which NADPH was being oxidized by the glutathione peroxidase/reductase system for the disposal of H2O2. This rate was determined in control cells and in catalase-i...

The study was designed to assess the relationship between red cell glutathione peroxidase and serum selenium as well as lipids and lipoprotein in HIV positive blood donors. Thirty five subjects consisting of twenty HIV positive blood donors with a mean age of 34±2.18 years and fifteen HIV negative blood donors with a mean age of 43±2.18 years controls were selected for this study. Erythrocyte g...

Purified enzymes were mixed to form a cell-free system that simulated the conditions for removal of hydrogen peroxide within human erythrocytes. Human glutathione peroxidase disposed of hydrogen peroxide (H2O2) at a rate that was only 17% of the rate at which human catalase simultaneously removed hydrogen peroxide. The relative rates observed were in agreement with the relative rates predicted ...

OBJECTIVE We aimed to determine the relationship between serum glutathione peroxidase and febrile seizure. MATERIALS & METHODS In this case-control study, 43 children with simple febrile seizure (case group) were compared with 43 febrile children without seizure (control group) in terms of serum glutathione peroxidase level, measured by ELISA method. This study was conducted in Qazvin Childre...

We conducted a comparative analysis of biochemical parameters for the ascorbate-glutathione cycle in non-transgenic and transgenic Nicotiana tabacum, plants cultivated heavy metal polluted soils. Transgenic had their genome bacterial acdS-gene encoding 1-aminocyclopropane-1-carboxylate deaminase (ACC-deaminase) enzyme. The introduction elevated concentrations copper, chromium, lead ions into so...

To the Editor: Ambade et al1 conducted an observational study to evaluate the potential serum biomarkers in subjects with COPD. The authors assessed superoxide dismutase-3, glutathione peroxidase, catalase, ceruloplasmin ferroxidase activity, C-reactive protein, and surfactant protein D in the serum of subjects with COPD and reported that ceruloplasmin ferroxidase activity and glutathione perox...

We determined selenium concentrations and activities of the selenoenzyme, glutathione peroxidase (EC 1.11.1.9), in the plasma and erythrocytes of 38 apparently healthy women. We determined selenium concentrations directly by polarized Zeeman-effect flameless atomic absorption spectroscopy. Within-run precision studies for the assays gave CVs of 5.6% for a mean erythrocyte selenium concentration...

INTRODUCTION Oxidative stress due to overproduction of reactive oxygen species and impairment in antioxidant defense mechanisms have been suggested as possible factors contributing to the pathogenesis of atherosclerosis in patients with end-stage renal disease. We compared glutathione levels, glutathione peroxidase and glutathione reductase activities, and total antioxidant capacity between pat...