It has recently been reported that O-linked β-N-acetyl glucosamine (O-GlcNAc) modification (a simple intracellular serine (Ser)/threonine (Thr)-linked monosaccharide) in human retinal microvascular endothelial cells (HRECs) is related to diabetic retinopathy (DR). During O-GlcNAcylation, O-GlcNAc is added to Ser and Thr residues. As the generation of reactive oxygen species (ROS) is one of the ...

UNLABELLED The genome of the white rot fungus Pycnoporus cinnabarinus includes a large number of genes encoding enzymes implicated in lignin degradation. Among these, three genes are predicted to encode glyoxal oxidase, an enzyme previously isolated from Phanerochaete chrysosporium The glyoxal oxidase of P. chrysosporium is physiologically coupled to lignin-oxidizing peroxidases via generation ...

A theoretical study of the mechanism and kinetics of the OH hydrogen abstraction from glyoxal and methylglyoxal is presented. Optimum geometries, frequencies, and gradients have been computed at the BHandHLYP/6-311++G(d,p) level of theory for all the stationary points, as well as for 12 additional points along the minimum energy path (MEP). Energies were obtained by single-point calculations at...

Tissue fixation in phosphate buffered formalin (PBF) remains the standard procedure in histopathology, since it results in an optimal structural, antigenic and molecular preservation that justifies the pivotal role presently played by diagnoses on PBF-fixed tissues in precision medicine. However, toxicity of formaldehyde causes an environmental concern and may demand substitution of this reagen...

The reaction of glyoxal with nucleophilic amino acids was monitored for β-casein as well as β-lactoglobulin. As predicted from previous experiments with hippuryl amino acids, a measurable decrease of arginine can be found in the thiol-free β-casein, while the lysine content remained almost unchanged. For β-lactoglobulin, the incubation with glyoxal led to a slight decrease in the lysine content...

Je re T. K oskinen Molecular Physics Laboratory. Department of Physics, POB 9, FIN-00014 University of Helsinki, Finland Z. Naturforsch. 53b, 386-392 (1998); received November 21, 1997 Proton Affinity, Gas Phase Basicity, Acid Dissociation Constant The structures of glyoxal bis(amidinohydrazone) (GBG) free base and glyoxal bis(amidinohydrazonium) monocation and dication were calculated quantum ...

Sesame cake and meal, byproducts of the sesame oil process industry mainly used as feed fertilizer, are often not optimally utilized wasted when material could be a high-quality protein source. This research primarily emphasizes preparation protein-based adhesive with urea glyoxal modification to use wood adhesive. The performance characterization modified (USP GUSP, respectively) were measured...

Up to 60 million people working indoors experience symptoms such as eye, nose and throat irritation, headache, and fatigue. Investigations into these complaints have ascribed the effects to volatile organic compounds (VOCs) emitted from building materials, cleaning formulations, or other consumer products. New compounds can result when the VOCs react with hydroxyl or nitrate radicals or ozone p...

Pseudomonas sp. MX-058 produces aldehyde oxidase catalysing glyoxal to glyoxylic acid. Two aldehyde oxidases (F10 and F13) were purified to homogeneity from Pseudomonas sp. MX-058. F10 and F13 had subunit structures, a heterotetramer and heteropentamer respectively. The N-terminal amino acid sequences of all subunits were highly homologous to amino acid sequences of the putative oxidoreductases...