Respiratory diseases provide an attractive target for gene silencing using small nucleic acids since the respiratory epithelium can be reached by inhalation therapy. Natural surfactant appears to facilitate the uptake and distribution of these types of molecules making aerosolized nucleic acids a possible new class of therapeutics. This article will review the rationale for the use of External ...

External guide sequences (EGSs) have successfully been used to inhibit expression of target genes at the post-transcriptional level in both prokaryotes and eukaryotes. We previously reported that EGS accessible and cleavable sites in the target RNAs can rapidly be identified by screening random EGS (rEGS) libraries. Here the method of screening rEGS libraries and a partial RNase T1 digestion as...

Editorial Topic 4 of the Renewable Energy Program of the Helmholtz Association focusses inter alia on the sustainable and cost-efficient use of geothermal reservoirs for base-load supply of heat and electricity by EGS. Current EGS projects show the limitations of such systems. Firstly, the localization of a potential reservoir prior to drilling still remains a major challenge. Secondly, the min...

The thermal stability and adsorption characteristics of three perfluorinated hydrocarbon compounds were evaluated under geothermal conditions to determine the potential to use these compounds as conservative or thermally-degrading tracers in Engineered (or Enhanced) Geothermal Systems (EGS). The three compounds tested were perfluorodimethylcyclobutane (PDCB), perfluoromethylcyclohexane (PMCH), ...

Net-zero-energy buildings (ZEBs) that contribute to making annual energy consumption balances zero are effective measures for reducing greenhouse gas (GHG) emissions in the construction sector. As application of ZEBs progresses, GHG during and manufacturing materials products (called EG) account a relatively large proportion overall emissions. This study aimed clarify EG as means by which formu...

Abstract In the present work, fully coupled dynamic thermo-hydro-mechanical (THM) model was employed to investigate advantage and disadvantages of supercritical CO2 (SCCO2) over water as geofluids. Low-temperature zone found in both SCCO2-enhanced geothermal system (EGS) water-EGS systems, but spatial expansion is higher water-EGS. Although, SCCO2 into rock matrix will help geo-sequestration, s...

Dolichos bean is grown in environments with short and long crop growth seasons. Development deployment of cultivars flowering time (FT) that matches prevailing season help maximize their productivity the to which they are targeted. Dependable knowledge on genetic basis FT enables use most appropriate selection strategy breed desired FT. We unraveled by detecting QTL controlling using SSR marker...

Rising fuel prices, changes in energy markets, and concern for the environment make it necessary to develop new solutions technologies. The development of technologies brings with need take risks associated unpredictable consequences, technological immaturity, other issues. However, without these elements, is not possible. In this study, installations related two different technologies—Enhanced...

A new approach for soft and self-sustainable oil-in-water emulsion gel systems (EGs) preparation based on a formulation with olive oil, sodium alginate, citric acid, calcium chloride, the combination of high-speed homogenization gelation induction by glucono-?-lactone addition is presented. Also, effect EG protein fortification incorporation quinoa concentrate (QC) flour physicochemical mechani...