The usefulness of bromcresol green for estimating albumin in urine was evaluated by comparison with the Laurell "rocket" technique. In contrast to the bromcresol green method applied for urinary albumin, rather doubtful results were obtained with conventional (Microzone) electrophoresis for albumin and with precipitation techniques for total protein estimation. Albumin estimation with bromcreso...

We have generated ‘green’ and ‘red’ BeWo cell lines: the green one constitutively expresses the green fluorescent protein (GFP) attached to histone H2B (Kimura & Cook, 2001), and the red one the red fluorescent protein tagged to the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase (Mit-DsRed; Bevis & Glick, 2002). Our quantitative assay for cell fusion (syncytial...

The fate of endocytosed membrane proteins and luminal contents is determined by a materials processing system in sorting endosomes. Endosomal retention is a mechanism that traps specific proteins within this compartment, and thereby prevents their recycling. We report that a sorting nexin SNX1, a candidate endosomal retention protein, self-assembles in vitro and in vivo, and has this property i...

Background: Chlamydomonas reinhardtii is a novel recombinant eukaryotic expression system with many advantages including fast growth rate, rapid scalability, absence of human pathogens and the ability to fold and assemble complex proteins accurately, however, obstacle relatively low expression level necessitates optimizing foreign gene expression in this system. The Green Fluorescent Protein (G...

The lack of heterotrimeric G-protein homologs in the sequenced genomes of green algae has led to the hypothesis that, in plants, this signaling mechanism coevolved with the embryophytic life cycle and the acquisition of terrestrial habitat. Given the large evolutionary gap that exists between the chlorophyte green algae and most basal land plants, the bryophytes, we evaluated the presence of th...

rhizosphere soil samples were collected from olive orchards of golestan, guilan, zanjan and qom provinces. fluorescent pseudomonad strains were isolated from the collected samples on king’s b medium and their phenotypic features characterized as based on the standard bacteriological methods. for all the tested strains oxidase reaction, fluorescent pigment, growth at 4?c and arginine dihydrolase...

We have cloned an unusual colourless green fluorescent protein (GFP)-like protein from Aequorea coerulescens (acGFPL). The A. coerulescens specimens displayed blue (not green) luminescence, and no fluorescence was detected in these medusae. Escherichia coli expressing wild-type acGFPL showed neither fluorescence nor visible coloration. Random mutagenesis generated green fluorescent mutants of a...