The kinetics of actin unfolding induced by guanidine hydrochloride has been studied. On the basis of obtained experimental data a new kinetic pathway of actin unfolding was proposed. We have shown that the transition from native to inactivated actin induced by guanidine hydrochloride (GdnHCl) passes through essential unfolding of the protein. This means that inactivated actin should be consider...

To improve our understanding of the contributions of different stabilizing interactions to protein stability, including that of residual structure in the unfolded state, the small sweet protein monellin has been studied in both its two variant forms, the naturally occurring double-chain variant (dcMN) and the artificially created single-chain variant (scMN). Equilibrium guanidine hydrochloride-...

The synthesis and characterization of some novel Pyrimidine derivatives have been presented. Acylation of resorcinol followed by nuclear prenylation with isoprene gives Chroman. Chroman on treatment with p-substituted benzaldehydes affords substituted chalcones. Pyrimidines have been prepared from chalcones by condensing with Guanidine hydrochloride in alkali medium. The structure of Pyrimidine...

3-Formyl-4-hydroxyphenylguanidine hydrochloride and its Schiff base copper(II), zinc(II), and iron(III) chelates were synthesized and their inhibitory activity against bovine beta-trypsin were determined. Syntheses of Schiff base metal chelates were carried out from 3-formyl-4-hydroxyphenylguanidine, various L-amino acids, and divalent metal acetate. Their structures were established on the bas...

Sections and freeze-fractured preparations showed an S layer on the surface of Pseudomonas-like strain EU2. Polyacrylamide gel electrophoresis of cell envelopes extracted with 1% sodium dodecyl sulfate (SDS) at room temperature showed three proteins (45K, 55K, and 110K). The 55K protein was identified as the S-layer protein. Incubation in 1.5 M guanidine hydrochloride removed the S layer from c...

Both MAT-B1 (nontransplantable) and MAT-Cl (xenotransplantable) ascites sublines of the 13762 rat mammary adenocarcinoma contain a major sialoglycoprotein (ASGP-1) with low electrophoretic mobility in dodecyl sulfate. ASGP-1 from each subline is rapidly purified by density gradient centrifugation of membrane fragments in cesium chloride containing 4 M guanidine hydrochloride. The products give ...

Thermal denaturation studies as a function of pH were carried out on wild-type iso-I-cytochrome c and three variants of this protein at the solvent-exposed position 73 of the sequence. By examining the enthalpy and T, at various pH values, the heat capacity increment (AC,,), which is dominated by the degree of change in nonpolar hydration upon protein unfolding, was found for the wild type wher...