Polysomes (S-20) from homogenates of guinea pig liver synthesized serum albumin and a precursor of the fourth component of guinea pig complement (C4) in vitro. The C4 precursor (pro-C4) accounted for approximately 0.2% and albumin 4% of the radiolabeled protein precipitable by trichloroacetic acid and not bound to polysomes. Pro-C4 is a single polypeptide chain (molecular weight 200,000) which ...

Livers of uninfected guinea pigs and of guinea pigs infected with Coxiella burneti were fractionated into smooth endoplasmic reticulum, rough endoplasmic reticulum (RER), pellet, and cell sap fractions. The ribonucleic acid (RNA) and protein of each fraction were determined, and the phosphorylase, glucose-6-phosphatase, and glucosyl transferase (glycogen synthetase) activities of each fraction ...

We are unable to confirm the observations reported by Cole and Favour that passive transfer of plasma fractions containing alpha globulin (IV + V or IV-10) from tuberculin-sensitive guinea pigs confers delayed sensitivity to tuberculoprotein upon normal animals. In guinea pigs with chronic cervical adenitis due to infection with group C hemolytic streptococci, injection of fraction IV + V and r...

The lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) isoenzyme distributions in tissues of the ICR mouse, Wistar rat, guinea pig and golden hamster were analyzed by histoelectrophoresis. Tissues obtained were as follows: liver, pancreas, stomach, small intestine, heart, femoral muscle, uterus, kidney, spleen, lymph node, cerebrum, spinal cord and erythrocyte. Histoelectrophoresis wa...

This paper describes stereospecific side chain hydroxylations in the biosynthesis of chenodeoxycholic acid by liver mitochondria and microsomes. The hydroxylation of 5/3-cholestane-3a,7a-diol was studied in subcellular fractions of rat, guinea pig, and rabbit livers. Incubation of SD-cholestane-3a,7n-diol resulted in hydroxylations at C-12, C-24, C-25, and C-26. The biosynthetic bile alcohols w...

Pheretima (family Megascolecidae) has been documented as a potent agent for the treatment of cough and breathing difficulty in traditional Chinese medicine for nearly 2000 years. The water extract of Pheretima was separated into three fractions of the ethanolic precipitate, the alkaline fraction and the acidic fraction. Among the three fractions, the acidic fraction showed the most potent spasm...

The present study was designed to investigate some histopathological changes produced by cimetidine on male reproductive organs (testes & epididymis) in mice and extended to examine wherethere using of aqueous suspension of powdered Nigella sativa seeds have ability to reduce this toxicity.Forty male mice were divided into 4 groups. The first group received 25 mg/kg B.W. of cimetidine orally on...

Palade, G.E. 1951. Intracellular localization of acid phosphatase: a comparative study of biochemical and histochemical methods. demonstration of the sites of activity of dehydrogenase systems with the electron microscope. A cytochemical study on the pancreas of the guinea pig: II. Functional variations in the enzymatic activity of microsomes. A cytochemical study on the pancreas of the guinea ...

Nigella sativa had been documented to possess many therapeutic functions in medicine but the least expected is sero-reversion in HIV infection which is very rare despite extensive therapy with highly active anti-retroviral therapy (HAART). This case presentation is to highlight the complete recovery and sero-reversion of adult HIV patient after treatment with Nigella sativa concoction for the p...

The cell-surface glycoprotein Mo1 is a member of the family of leukocyte cell adhesion molecules (Leu-CAMs) that includes lymphocyte function-associated antigen 1 (LFA-1) and p150,95. Each Leu-CAM is a heterodimer with a distinct alpha subunit noncovalently associated with a common beta subunit. Leu-CAMs play crucial roles in cell-cell and cell-matrix interactions. We describe the isolation and...