Protoplasts were isolated from five species of Gossypium. · Protoplast yield and viability were affected by incubation conditions, osmolarity, purification procedures, and cell source. Using an optimized procedure, highly viable protoplasts were isolated from cell suspensions, callus cultures, and leaf tissue of G. hirsutum, G. arboreum, G. k/otzschianum, G. harlmessii, and G. herbaceum. Protop...

Cationic dye - basic red 2 (BR2) was removed from aqueous solutions by sulfuric acid activated waste Gossypium hirsutum seeds (WGSAB). The main and interactive effects of five process variables such as, adsorbent dose (1.25 – 5 g/L), initial dye concentration (100–300 mg/L), contact time (1–3 h),  pH (2 – 12) and temperature (20 – 400C) were investigated via  response su...

We developed a set of genetic markers specific to the A and D genome types of cotton using representational difference analysis (RDA). These markers produce amplification products with genomic DNA from allotetraploid cotton Gossypium hirsutum. One of the markers is a polymorphic amplified restriction fragment (PARF) - a sequence found in both A and D genomes but differently flanked by restricti...

Salinity is one of the major environmental factors limiting plant growth and productivity. An H(+)-PPase gene, TsVP from Thellungiella halophila, was transferred into cotton (Gossypium hirsutum) in sense and antisense orientations under control of the cauliflower mosaic virus (CaMV) 35S promoter. Southern and Northern blotting analysis showed that the sense or antisense TsVP were integrated int...

Cell-free enzyme particles from mung beans (Phaseolus aureus) or cotton (Gossypium hirsutum L.) fibers catalyze the incorporation of mannose from GDP-[(14)C]mannose and N-acetylglucosamine from UDP-[(3)H]-N-acetylglucosamine into polyprenyl-type lipids. These lipids have been synthesized and purified and the lipid moieties compared to each other as well as to dolichyl phosphate and to lipids is...

We tested the behavioral responses of ovipositing females and natal larvae of two sibling species, a generalist Helicoverpa armigera (Hübner) and a specialist Helicoverpa assulta (Guenée), to odor sources emitted from different combinations of six plant species (tobacco, Nicotiana tabacum; hot pepper, Capsicum annuum; tomato, Solanum esculentum; cotton, Gossypium hirsutum; peanut, Arachis hypog...

Mg(2+)-ATP-dependent H(+)-translocation has been studied in membrane vesicles derived from the roots of Gossypium hirsutum L. var. Acala San Jose 2. Establishment of a positive membrane potential was followed by measuring SCN(-) accumulation; establishment of DeltapH across the vesicle membranes by measuring quinacrine fluorescence quenching. High specificity for ATP was shown, and H(+)-translo...

Trichogramma Westwood (Hymenoptera: Trichogrammatidae) cause high mortality rates in the potentially resistant pest species, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), and are considered integral to the resistance management plan for Bacillus thuringiensis transgenic cotton, Gossypium hirsutum L., production in the Ord River Irrigation Area (ORIA), WesternAustralia.Measured as perc...

Four rates of methyl bromide (Mbr) (16.8, 33.6, 67,2, and 134.4 kg a.i./ha) and one rate of 1,3-dichloropropene (1,3-D) (28.1 liters a.i./ha) were evaluated over 2 years for control of Hoplolaimus columbus on cotton. All nematicide treatments were applied through a tarpless subsoiler-bedder prior to planting cotton, Gossypium hirsutum cv. Dehapine 90. Nematode population densities were monitore...