The hemopoietic growth factor interleukin 3 (IL-3) supports the survival and proliferation of multipotent and committed progenitor cells in vitro. To elucidate the molecular mechanisms triggered by IL-3 we studied the expression of cell cycle-related genes in a recently established human IL-3-dependent clone (M-07e). No changes in the level of expression of early (c-myc), mid (ornithine decarbo...

The most recently replicated portion of the bacterial and T4 phage chromosome, selectively labeled by an extremely short radioactive pulse, can be isolated, after denaturation, as short DNA chains with an average sedimentation coefficient of 7-11S.1-3 This supports an hypothesis of discontinuous DNA chain growth, by which short stretches of DNA are synthesized at the replicating point and subse...

The binding of Sodium n-dodecyl sulphate (SDS) to histone H3 was studied in the pH range 3.2-10 by equilibrium dialysis at 27? and 3 7 ?c .T he binding data have been used to obtain the Gibbs free energy of interaction using a theoretical model of the Wyman binding potential; and the enthalpy of interaction from the temperature dependence of theequilibriumconstantsfronr theVan't Hoff re1ati...

the role of endogenous histamine and h1, h2 and h3 central receptors on food intake in broiler chickenswas investigated. for this purpose, a probe was used to manipulate the concentration of endogenoushistamine by intracerebroventricular (icv) injection of thioperamide, an h3 receptor antagonist, and r-α-methylhistamine, an h3 receptor agonist and subsequently the effects of brain histaminergic...

Histone methylation patterns in the human genome, especially in euchromatin regions, have not been systematically characterized. In this study, we examined the profile of histone H3 methylation (Me) patterns at different lysines (Ks) in the coding regions of human genes by genome-wide location analyses by using chromatin immunoprecipitation linked to cDNA arrays. Specifically, we compared H3-KM...

Background: Many in vitro studies suggested that choline incorporation into many types of tumors is related to cell proliferation. Whether in vivo choline incorporation is related also to cell proliferation or not was my question. Therefore, the aim of this pilot study was to investigate the relationship of in vivo [methyl- 14C]-choline incorporation and in vivo [methyl-3H]- thymidine incorpora...

It is an established concept that in any given cell or tissue of a multicellular organism, only a part of the total genetic information is used. Although the exact mechanism has not been elucidated, it is presumed that reversible and irreversible inactivations of genetic loci with respect to RNA synthesis are the controlling factors. Hoyer and McCarthy' have demonstrated with DNA/RNA hybridizat...

Objective To assess the risk of type 2 diabetes (T2D) in women with polycystic ovary syndrome (PCOS) relation to body mass index (BMI) and hyperandrogenic (HA) PCOS phenotype. Design Population-based cohort study. Setting Data from six Swedish national registers, participants being followed for a maximum 19 years. Patient(s) All an International Statistical C...