Gloriosa superba L. a medicinally important monocot, member of the family Liliaceae, was investigated for callusing, direct regeneration and induction of hairy root using soil bacterium Agrobacterium rhizogenes. Friable callus was initiated from corms on basal MS medium amended with 2,4-D (1 mg L) and kinetin (1 mg L). Callus generated from such effective combinations were co-cultivated with th...

Elite genotypes from high yielding variety of Artemisia annua L. (Jeevanraksha, CIMAP Lucknow) were regenerated form nodal explants and maintained in MS culture medium with BAP and NAA. The leaves of tissue culture grown plants were inoculated with Agrobacterium rhizogenes strain LBA9402 containing the selective marker gene NPT II. Hairy roots emerged from the leaves, 8-10 days after inoculatio...

A kinetic metabolic model describing Catharanthus roseus hairy root growth and nutrition was developed. The metabolic network includes glycolysis, pentose-phosphate pathway, TCA cycle and the catabolic reactions leading to cell building blocks such as amino acids, organic acids, organic phosphates, lipids and structural hexoses. The central primary metabolic network was taken at pseudo-steady s...

Rosmarinic acid is one of the main active components of Coleus blumei and is known to have numerous health benefits. The pharmacological significance of rosmarinic acid and its production through in vitro culture has been the subject of numerous studies. Here, the ability of different tissues to accumulate rosmarinic acid and sustainability in production over long cultivation have been tested. ...

Elicitation with middle-viscous chitosan (30 mg/50 mL) significantly stimulated silymarin synthesis in Silybum marianum hairy root cultures. The root cultures established by infection with Agrobacterium rhizogenes AR15834 showed a potential for production of silymarin. Elicitation with medium molecular weight of chitosan (0, 5, 10, 20, and 30 mg/50 mL) was used in order to improve silymarin pro...

Hairy roots are plants genetically transformed by Agrobacterium rhizogenes, which do not produce shoots and are composed mainly by roots. Hairy roots of Ophiorrhiza mungos Linn. are currently gaining interest of pharmacologists, since a secondary product of their metabolism, camptothecin, is used in chemotherapy. To optimize the production of valuable secondary metabolites it is necessary to un...

E-mail: [email protected] ABSTRACT: Andrographolide is one of the main active principles in Andrographis paniculata .It exhibits enormous pharmacological effects. Due to this immense value it required more level in pharmaceutical to satisfy the global need. Because it present in a very low level in normal regenerated plant of Andrographis paniculata. Micro propagation is a proven method f...

background: trigonella foenum-graecum l. commonly known as fenugreek is a rich source of important medicinal metabolite, i.e. trigonelline. objective: in this study, hairy roots culture as a novel method for trigonelline production was evaluated. methods: for optimizing the hairy roots culture of trigonella foenum-graecum, three strains of agrobacterium rhyzogenes (atcc15834, msu440 and k599) v...