OBJECTIVE To detect hepatitis C virus (HCV) antibodies in seronegative donors by disruption of the immune complexes (ICs). SUBJECTS AND METHODS HCV antibody detection was carried out on 600 seronegative donors following an IC dissociation assay. Reverse transcription polymerase chain reaction (RT-PCR) was then performed on the positive results. RESULTS Nine of the 600 samples (1.5%) were po...

Persistent hepatitis C virus (HCV) infection frequently causes hepatocellular carcinoma. However, the mechanisms of HCV-associated hepatocarcinogenesis and disease progression are unclear. Although the human hepatoma cell line, HuH-7, has been widely used as the only cell culture system for robust HCV replication, we recently developed new human hepatoma Li23 cell line-derived OL, OL8, OL11, an...

background: hepatitis c virus (hcv) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. therefore, designing new and effective therapeutics is of great importance. the aim of the current study was to construct a dna vaccine containing structural proteins of hcv and evaluation of its expression in a eukaryotic system. methods: struct...

Molecular diagnostics based on reverse transcription (RT)-PCR are routinely complicated by the lack of stable internal controls, leading to falsely negative results. We describe a strategy to produce a stable competitive internal control (CIC) based on a Qbeta phage derivative (recombinant Qbeta [rQbeta]) bearing primers KY78 and KY80, which are widely used in the detection of hepatitis C virus...

Hepatitis C virus (HCV) has infected 3% of the population worldwide and 20% of the population in Egypt. HCV infection can lead to hepatocellular carcinoma and death. The presently available treatment with interferon plus ribavirin, has limited benefits due to adverse side effects. Seaweeds have become a major source of new compounds to treat viral diseases. This work aimed to study the effect o...

conclusions according to the results, it was found that hcv high prevalent genotype in zanjan is subtype 3a. analysis of the results provides identification of certain hcv genotypes, and these valuable findings could affect the type and duration of the treatment. results samples indicated 216 bp bands on 2% agarose gel. analyses of the results demonstrated that the most dominant subtype was 3a ...

Amplification of RNA by the polymerase chain reaction (PCR) is normally a two-step process requiring separate enzymes and buffer conditions. We describe a combined reverse transcription-PCR (RT-PCR) assay for hepatitis C virus (HCV) RNA amplification in which a single enzyme and buffer condition are used. In this assay, both the RT and PCR steps are carried out with the thermoactive DNA polymer...

The prevalence, virological and epidemiological aspects of the hepatitis C virus (HCV) and the hepatitis B virus (HBV) infections vary among hemodialysis patients in different countries. Aiming at analyzing these aspects of HCV and HBV infections in hemodialysis patients in Belo Horizonte, MG, Brazil, we studied three hemodialysis units including 434 patients. Serology was used to detect anti-H...

Background: Hepatitis B and C virus (HBV HCV) infections are major global health issues the leading causes of liver cirrhosis hepatocellular carcinoma. Objectives: The aim this study was to investigate prevalence HBV HCV in couples attending a premarital screening program Zakho city, Kurdistan region, Iraq. Methods: This cross-sectional carried out Region Iraq, between January October 2019. Dur...

BACKGROUND Occult hepatitis C virus (HCV) infection is a new entity described by the presence of HCV-RNA in liver biopsy and/or peripheral blood mononuclear cell (PBMC) specimens, and undetectable levels or absence of HCV-RNA and in the absence or presence of anti HCV antibodies in plasma by current laboratory methods. OBJECTIVES To evaluate the detection of HCV-RNA in PBMC specimens of the l...