levofloxacin is one of the fluroquinoline antibiotic groups, which affect on controlling infections, especially in reproductive organs. it has therapeutic use in numerous countries, but little information exists on the effects of levofloxacin on spermatogenesis when it is used for infectious treatment. the current study was designed to determine whether levofloxacin influences testis tissue and...

Flowering plants have evolved a unique reproductive process called double fertilization, whereby two dimorphic female gametes are fertilized by two immotile sperm cells conveyed by the pollen tube. The two sperm cells are arranged in tandem with a leading pollen tube nucleus to form the male germ unit and are placed under the same genetic controls. Genes controlling double fertilization have be...

Objective: To report a successful pregnancy after transfer of embryos derived from oocytes activated by calcium ionophore after intracytoplasmic sperm injection (ICSI) with round-headed sperms and aneuploid screening using aCGH. Design: Case report Setting: Private IVF clinic. Patient: A 28-year-old patients and her 31-year-old husband, diagnosed with 100% globozoospermia, underwent ICSI, oocyt...

The aim of this comparative clinical study was to examine whether the fertilizing potential of frozen-thawed testicular sperm in the most severe cases of hypospermatogenesis is reduced compared with fresh testicular sperm. The results could determine the necessity of using fresh testicular sperm cells, which mandates involving the spouse by performing simultaneous in vitro fertilization intracy...

abstract background: sperm cells extracted from testes (tese) have poor chromatin quality and motility. various substances are used in the laboratory to increase sperm motility and improve the art outcomes; however, there are few research which considered improving both sperm motility and chromatin quality. objective: the aim of this investigation was to evaluate the improvement of the testicul...

Seminolipid (sulphogalactosylalkylacylglycerol), the glycolipid that is specific for mammalian germ cells, is located exclusively in the outer leaflet of the sperm plasma membrane. In this study the lateral distribution of seminolipid on sperm heads has been investigated by indirect immunofluorescence labelling and detection with digital imaging fluorescence microscopy. In freshly ejaculated sp...

Sperm dimensions and the question of whether X and Y chromosome-bearing sperm differ in size or shape has been of great interest, especially for the development of alternative methods to sort or classify sperm cells. The aim of the present study was to evaluate possible differences in the shape and size of the sperm head between X and Y chromosome-bearing sperm by atomic force microscopy (AFM)....

The selection of sperm cells possessing normal morphology and motility is crucial for many assisted reproductive technologies (ART), especially for intracytoplasmic sperm injection (ICSI), as sperm quality directly affects the probability of inducing healthy pregnancy. We present a novel platform for real-time quantitative analysis and selection of individual sperm cells without staining. Towar...