نتایج جستجو برای: hemi nested rt
تعداد نتایج: 92535 فیلتر نتایج به سال:
چکید ه سابقه و هدف آنتی ژن rhd در ایجاد بیماری های همولیتیک نوزادان نقش مهمی دارد و از آن جایی که تعیین rhd جنین با استفاده از روش های تهاجمی مانند آمنیوسنتز، خطراتی را برای مادر و جنین به دنبال خواهد داشت، در این پژوهش تلاش گردید تا ژنوتایپ rhd جنین از dna آزاد جنینی موجود در سرم مادران باردار، به صورت غیر تهاجمی و با استفاده از روش hemi-nested pcr تعیین گردد. مواد وروش ها مطالعه انجام...
AbstractDengue virus (DENV) is a serious threat to public health. Having reliable estimates of the burden of dengue is important to inform policy and research, but surveillance systems are not designed to capture all symptomatic DENV infections. We derived the rate of reporting of dengue by comparing active surveillance of symptomatic DENV infections in a prospective community-based seroepidemi...
زمینه و هدف: واکنش زنجیره پلیمراز(pcr) رایج ترین تکنیک در عرصه زیست شناسی مولکولی است که برای تکثیر یک توالی اختصاصی اسید نوکلئیک به کار می رود. پرایمرهای دژنره دارای توانایی تکثیر توالی های وابسته اما متفاوت هستند. هدف مطالعه حاضر استفاده از دو جفت پرایمر دژنره در ترکیب با hemi nested pcr برای تشخیص ناحیه v3-loop ژن پوشش طیف وسیعی از ساب تایپ های ویروس hiv-1 است. مواد و روش ها: در مطالعه تجربی...
Control of rabies requires a consistent supply of dependable resources, constructive cooperation between veterinary and public health authorities, and systematic surveillance. These are challenging in any circumstances, but particularly during conflict. Here we describe available human rabies surveillance data from Iraq, results of renewed sampling for rabies in animals, and the first genetic c...
INTRODUCTION The burden of rotavirus gastroenteritis is greatest in India and other developing countries. With the availability of two licensed vaccines and a number of additional vaccines in various stages of development and trial, analysis of detailed clinical information is essential for the development of a uniform method of severity assessment. METHODS Diarrhoeal stool samples from 1001 ...
Parietal cortical areas are involved in sensori-motor transformations for their respective contralateral hemifield/body. When arms of the subjects are crossed while their gaze is fixed straight ahead, vision of the hand is processed by the hemisphere ipsilateral to the arm position and proprioception of the arm by the contralateral hemisphere. It induces interhemispheric transfer and remapping....
PURPOSE Reverse-transcriptase PCR (RT-PCR) assays for carcinoembryonic antigen (CEA) have been described to identify lymph node micrometastases. These assays are not quantitative and can be confounded by false-positive results. The purpose of this study was to determine whether quantification of CEA in lymph nodes could more readily identify clinically relevant groups. EXPERIMENTAL DESIGN Spe...
Rabies in bats is considered enzootic throughout the New World, but few comparative data are available for most countries in the region. As part of a larger pathogen detection program, enhanced bat rabies surveillance was conducted in Guatemala, between 2009 and 2011. A total of 672 bats of 31 species were sampled and tested for rabies. The prevalence of rabies virus (RABV) detection among all ...
OBJECTIVE To detect the presence of dengue virus in larval forms of Aedes aegypti and to associate vector presence with rainfall and incidence of disease. METHODS Eighteen households were randomly selected for egg collection in a neighborhood of the city of Boa Vista, Roraima, in Northern Brazil. Two oviposition traps were installed per home, and removed after one week. This was repeated on a...
A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.
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