Detection of protein-protein interactions (PPIs) is important for understanding numerous processes in mammalian cells; however, existing PPI detection methods often give significant background signals. Here, we propose a novel PPI-detection method based on kinase-mediated growth induction of mammalian cells. In this method, target proteins are fused to the intracellular domain of c-kit (c-kit I...

BACKGROUND A mutation in the sarcomeric gene coding for the myosin-binding protein C gene has been identified in a colony of Maine Coon cats with hypertrophic cardiomyopathy (MyBPC3-A31P mutation). However, the close correlation between genotype and phenotype (left ventricular hypertrophy [LVH] and dysfunction) has never been assessed in a large population, particularly in heterozygous (Hetero)...

We theoretically investigate the thermodynamic stability of a normal-state Bose-Fermi mixture, with tunable pairing interaction $-U_{\rm BF}<0$ associated hetero-nuclear Feshbach resonance, as well weak repulsive Bose-Bose $U_{\rm BB}\ge 0$. Including strong hetero-pairing fluctuations former within self-consistent $T$-matrix approximation, latter mean-field level, we calculate compressibility ...

Citrus cultivation involves the widespread practice of grafting, which has a significant impact on citrus development and fruit quality yield. However, understanding effect flavonoid compounds after different rootstock grafting have been limited. Flavonoid compounds, found at highest levels in peels, contribute to improving nutritional value. In this study, scion-rootstock interaction was inves...

We report here, the results of molecular dynamics simulation of p-doped (Ga-face)GaN over n-doped (Siface)(0001)4H-SiC hetero-epitaxial material system with one-layer each of Ga-flux and (Al-face)AlN, as the interface materials, in the form of, the total Density of States (DOS). It is found that the total DOS at the Fermi-level for the heavily p-doped (Ga-face)GaN and ndoped (Si-face)4H-SiC het...

G protein gated inward rectifier potassium (GIRK) channels are gated by direct binding of G protein beta-gamma subunits (Gβγ), signaling lipids, and intracellular Na(+). In cardiac pacemaker cells, hetero-tetramer GIRK1/4 channels and homo-tetramer GIRK4 channels play a central role in parasympathetic slowing of heart rate. It is known that the Na(+) binding site of the GIRK1 subunit is defecti...

Cyclodextrin poly-functionalization has fueled progress in their use multiple applications such as enzyme mimicry, but also the polymer sciences, luminescence, sensors or for biomedical applications. However, regioselective access to a given pattern of functions on β-cyclodextrin is still very limited. We uncover new orienting group, thioacetate, that expands toolbox available cyclodextrin poly...

Fe2V4O13 is prepared and characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR), UV-diffuse reflectance spectroscopy (UV-DRS), high resolution scanning electron microscopy (HR-SEM) using energy dispersive X-ray spectroscopy (EDX) analysis. The hetero-Fenton catalyst can be used to mineralize Methyl Orange (MO) under UV-A light. The mineralization rate is influenced by hydr...

MOTIVATION While there are numerous programs that can predict RNA or DNA secondary structures, a program that predicts RNA/DNA hetero-dimers is still missing. The lack of easy to use tools for predicting their structure may be in part responsible for the small number of reports of biologically relevant RNA/DNA hetero-dimers. RESULTS We present here an extension to the widely used ViennaRNA Pa...

A family of furoquinolines were efficiently obtained through a tandem acetalization/cycloisomerization process catalyzed by (5 mol%) silver imidazolate polymer and triphenylphosphine, and diversity was brought by the use of 7 different alcohol groups. From these furoquinolines, 3 examples of reduced derivatives could be obtained (d.r. up to 94 : 6), 10 different spiroketal derivatives by hetero...