Cationic latex particles with surface amino groups were prepared by a multistep batch emulsion polymerization. In the first one, two or three steps, monodisperse cationic latex particles to be used as the seed were synthesized. In the third and fourth steps, the amino-functionalizedmonomer aminoethylmethacrylate hydrochloride was used to synthesize the final functionalized latex particles. Thre...

A high performance liquid chromatography (HPLC) paired with UV-vis detection method to determine ascorbic acid and its oxidation product, dehydroascorbic acid, in human plasma was developed. Ascorbic acid in human plasma was extracted and stabilized using 10% metaphosphoric acid, and was analyzed by a Symmetry C18 column with 5 mM Hexadecyltrimethylammonium bromide and 50 mM KH2PO4 solution as ...

A simple, economical, and sensitive capillary electrophoresis (CE) method integrated with capacitively coupled contactless conductivity detection was developed for the determination of metal ions such as K+, Na+, Mg2+, Sr2+, Ca2+ in drinking water. 18-Crown-6 ether and Hexadecyltrimethylammonium Bromide (CTAB) were employed as complexing reagents. The effects of electrolyte additives, citric ac...

The toxicities of three synthetic surfactants to the marine macroalga, Ulva lactuca, have been examined by monitoring chlorophyll a fluorescence quenching. The anionic surfactant, sodium dodecyl sulphate (SDS), exerted no measurable toxicity over the concentration range 0–10 mg L, while presence of the non-ionic surfactant, Triton X-100 (TX), elicited a small reduction in photochemical efficien...

A method is used for preparing gold NRs with aspect ratios ranging from 1.5 to 10 for which the surface plasmon absorption maxima are between 600 and 1300 nm. This method has been adapted from a previously published seed-mediated growth method (Jana et al. Adv. Mater. 2001, 13, 1389). The disadvantages and limitations of the earlier method (i.e., formation of noncylindrical NRs, φ-shaped partic...

Isolation of nucleic acids from Chlorella is difficult, given the chemically complex nature of their cell walls and variable production of metabolites. Immobilization of microalgae in polymers adds additional difficulty. Here, we modified, amended, and standardized methods for isolation of nucleic acids and compared the yield of DNA and RNA from free-living and encapsulated microalgae C. soroki...

Nigella sativa L. (Ranunculaceae) seeds (black cumin) that contain high oil, terpene, protein, alkaloids are an economically important for medicine and food industry. Lack of an efficient DNA isolation procedure is a limiting factor for any molecular studies of these plant. We have used a genomic DNA isolation protocol for Nigella sativa seeds based on a hexadecyltrimethylammonium bromide (CTAB...

Melia dubia Cav. of family Meliaceae is a fast growing, high value tree species native to India. Isolating DNA from matured dried leaves of M. dubia was difficult due to accumulation of secondary metabolites, majorly polyphenolics, which resulted in dark brown to black colour of the pellet. In this study, a modified STE-(Sucrose, Tris-HCl and Ethylene Diamine Tetra Acetic Acid) CTAB (hexadecylt...

The accurate detection of pathogens in environmental matrices, such as sediment, is critical in understanding pathogen fate and behavior in the environment. In this study, we assessed the usefulness of methods for the detection and quantification of Vibrio spp. and norovirus (NoV) nucleic acids in sediment. For bacteria, a commonly used direct method using hexadecyltrimethylammonium bromide (CT...

Uniformly sized and shape-controlled nanoparticles are important due to their applications in catalysis, electrochemistry, ion exchange, molecular adsorption, and electronics. Several ferric phosphate hydroxide (Fe4(OH)3(PO4)3) microstructures were successfully prepared under hydrothermal conditions. Using controlled variations in the reaction conditions, such as reaction time, temperature, and...