oDays High-performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light-scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soyheans subjected to storage at high moisture conten...

A major protein-bound ligand in the serum of chronic hemodialysis patients was isolated from heat-deproteinized uremic serum by "high-performance" liquid chromatography (HPLC). The isolated compound was identified as 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid by use of liquid secondary-ion mass spectrometry and gas chromatography-mass spectrometry. On HPLC, an authentic sample of this co...

Submit Manuscript | http://medcraveonline.com Abbreviations: HPLC: High Pressure Liquid Chromatography; DAD: Diode Array Detector; RSD: Relative Standard Deviation; RE: Relative Error; CPF: Cupressuflavone; AMF: Amentoflavone; CNC: Cupressus from North Coast, Egypt; CFU: Cupressus from Future University, Egypt; MS: Murashige and Skoog medium; PGRS: Plant Growth Regulators; NAA: β-Naphthalene Ac...

Reversed phase high pressure liquid chromatography (HPLC) solvent systems have been developed for the separation of intermediates in the formation of bile acids and bile acid conjugates from cholesterol. Four different mobile phases (water-methanol, 10 mM acetate buffer (pH 4.37)-methanol, 30 mM trifluoroacetic acid (pH 2.9 with triethylamine)-methanol, and 50 mM potassium phosphate buffer (pH ...

The paper focuses on the analysis and detection of electroactive compounds using high-performance liquid chromatography (HPLC) combined with electrochemical detection (EC). The fabrication and utilization of electrochemically treated carbon fiber microelectrodes (CFMs) as highly sensitive amperometric detectors in HPLC are described. The applied pretreatment procedure is beneficial for analytic...

Methylhydrazine oxidation promoted by horseradish peroxidase-H2O2 or ferricyanide led to the generation of high yields of methyl radicals and to the formation of 7-methylguanine and 8-methylguanine upon interaction with calf thymus DNA. Methyl radicals were identified by spin-trapping experiments with alpha-(4-pyridyl-1-oxide)-N-tert-butyl nitrone and tert-nitrosobutane. The methylated guanine ...

During the past decade, technological innovations based on new knowledge of the principles underlying separations processes have transformed column liquid chromatography, the oldest chromatographic technique, into a sophisticated tool for rapidly achieving the most difficult of separations and analyses. Applications of this modern version called High Performance Liquid Chromatography (HPLC) are...

High performance liquid chromatography (HPLC) identifies compounds in a sample and quantifies their concentration. The instrument consists of an injector that introduces μL to mobile phase carries the analytes across column filled with adsorbing substance. adsorb desorb at different rates as they flow down are thereby separated. Detectors record signal proportional concentration analyte phase. ...

This work proposes a procedure to estimate the measurement uncertainty for the quantification of cefazolin by high-performance liquid chromatography (HPLC) and microbiological assay (bioassay). HPLC method was performed according to United States Pharmacopeia (USP) and bioassay according to Brazilian Pharmacopeia (FB). The quantification of cefazolin by high-performance liquid chromatography wa...