A morphologically distinct isolate of Cladosporium sphaerospermum from a North American patent collection, referenced as Cladosporium lignicola in the patent, was examined. Generic affinity was confirmed by scanning electron microscopic examination of conidiogenous loci and conidial hila. Species identity as C. sphaerospermum was indicated by DNA sequence data derived from actin and translation...

Signature-tagged mutagenesis is a functional genomics approach to identify bacterial virulence genes by simultaneously screening multiple mutants in a single host animal. Avirulent (attenuated) mutants are identified by negative selection (failure to colonize the host). The method was recently developed to investigate Salmonella typhimurium in a mouse model of human typhoid fever. We modified t...

Median sternotomy due to its technical simplicity and excellent exposure of the heart, great vessels and pulmonary hila is the most common incision performed in cardiothoracic surgery worldwide [1]. Originally described by Julian more than 100 years ago and re-induced by Milton in 1957, median sternotomy replaced gradually thoracotomy or bilateral transverse sternothoracotomy (clamshell incisio...

rhs work W U I c!onc 31 hila f h t f i n t c ~ i i ~ f i o r 1% us on \ahbuti<c,! system design and scheduling algorithm and avoiding poor or erroneous choices. The scheduling theory literature is so vast that we can’t pretend to be comprehensive, but this article does present a minimum set of results and their implications. The set includes Jackson’s rule, Smith’s rule, McNaughton’s theorem, L...

Virulence factors generally enhance a pathogen's fitness and thereby foster transmission. However, most studies of pathogen fitness have been performed by averaging the phenotypes over large populations. Here, we have analyzed the fitness costs of virulence factor expression by Salmonella enterica subspecies I serovar Typhimurium in simple culture experiments. The type III secretion system ttss...

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a major intestinal pathogen of both humans and animals. Salmonella pathogenicity island 1 (SPI-1)-encoded virulence genes are required for S. Typhimurium invasion. While oxygen (O2) limitation is an important signal for SPI-1 induction under host conditions, how the signal is received and integrated to the central SPI-1 regulatory syst...

During our dissection classes, we observed a lobulated spleen with multiple hila and fissures. The spleen presented 4 hila and 5 deep fissures. The hila were seen on the visceral surface. The fissures extended into the substance of the spleen from superior border, inferior border and the visceral surface. Because of these fissures, the spleen appeared to be lobulated, having seven distinct lobe...