Address correspondence to Martin A. Kennedy, Department of Pathology & Carney Centre for Pharmacogenomics, University of Otago, Christchurch, PO Box 4345, Christchurch, New Zealand. E-mail: [email protected].

Although the thermophilic bacterium Thermus aquaticus grows optimally at 70 degrees C and cannot grow at moderate temperatures, its DNA polymerase I has significant activity at 20-37 degrees C. This activity is a bane to some PCRs, since it catalyzes non-specific priming. We report mutations of Klentaq (an N-terminal deletion variant) DNA polymerase that have markedly reduced activity at 37 deg...

The polymerase chain reaction (PCR) is widely used for applications which require a high level of specificity and reliability, such as genetic testing, clinical diagnostics, blood screening, forensics and biodefense. Great improvements to PCR performance have been achieved by the use of Hot Start activation strategies that aim to prevent DNA polymerase extension until more stringent, higher tem...

Designing reliable CMOS chips involve careful circuit design with attention directed to some of the potential reliability problems such as electromigration and hot carrier e ects. This paper considers logic synthesis to handle electromigration and hot carrier degradation early in the design phase. The electromigration and the hot carier e ects are estimated at the gate level using signal activi...

Objective(s): Use of a positron emission tomography (PET)/single-photonemission computed tomography (SPECT) system facilitates the simultaneousacquisition of images with fluorine-18 fluorodeoxyglucose (18F-FDG) andtechnetium (99mTc)-tetrofosmin. However, 18F has a short half-life, and 511keV Compton-scattered photons are detected in the 99mTc energy window.Therefore, in this study, we aimed to ...

This work contributes to the development of ontology-based user models, devised as overlays over conceptual hierarchies derived from domain ontologies. We tackle the problem of propagation of user interests in such a conceptual hierarchy. In addition to accounting for the hierarchical structure of the domain and the type and amount of feedback provided by the user, the principal contributions i...

This paper describes the Intelius-NYU 2012 system for the KBP Cold Start task. The Cold Start task can be decomposed into two subtasks: slot filling and entity linking. For slot filling, we focus on the adaptation of the NYU 2011 regular slot filling system to the Cold Start task. For entity linking, we apply Intelius‘s commercial conflation engine to link person and organization entities with ...

When a new user registers to a recommender system service, the system does not know her taste and cannot propose meaningful suggestions (cold-start problem). This preliminary work attempts to mitigate the cold-start problem using the profile picture of the user as a sole information, following the intuition that a correspondence may exist between the pictures that people use to represent themse...