Analytical methods including HPLC, UPLC and HPTLC are presented for the determination of major alkaloid and triterpene saponins from the roots of Caulophyllum thalictroides (L.) Michx. (blue cohosh) and dietary supplements claiming to contain blue cohosh. A separation by LC was achieved using a reversed phase column, PDA with ELS detection, and ammonium acetate/acetonitrile gradient as the mobi...

Six natural polyphenolic compounds, brevifolin carboxylic acid, brevifolin, ellagic acid, methyl gallate, gallic acid and protocatechuic acid have been isolated from the methanol extract of the whole plant of Erodium cicutarium (L.) L.'Hérit. (Geraniaceae). Structures were determined by conventional methods of analysis and confirmed by MS and NMR spectral analysis. The distribution of these com...

In this study, we have chosen HPTLC as a method of analysis to develop a standard procedure based on fingerprinting characteristics for the evaluation of homoeopathic formulations. A simple and accurate HPTLC method has been developed for the quantification of Arjunolic acid and fingerprinting of the in-house mother tincture considered here to be a standard with that of different marketed sampl...

In this study, we have chosen HPTLC as a method of analysis to develop a standard procedure based on fingerprinting characteristics for the evaluation of homoeopathic formulations. A simple and accurate HPTLC method has been developed for the quantification of BacosideA and fingerprinting of the in-house mother tincture considered here to be a standard with that of different marketed samples av...

Curcuma longa L. rhizomes are widely used by the traditional medical practitioners for curing various diseases. The rhizomes of C. longa L. is commonly known as Haldi or Turmeric. Haldi is of special importance to human with the discovery that its rhizome powder, when added to various food preparations, preserves their freshness and imparts a characteristic flavors. The Indian system of medicin...

coronary artery disease (cad) is the most important cause of mortality and morbidity in the world. the knowledge of major risk factos can be useful in prevention of cad. there is no known major risk factof in many patients with myocardial infarction (mi). the dermatoglyphic pattern in patients with myocardial infarction is an interesting matter and little information is available about this rel...

A radial "high-performance" thin-layer chromatographic (HPTLC) method is described by which the percentages and ratios of phosphatidylinositol, sphingomyelin, lecithin, phosphatidylethanolamine, phosphatidylglycerol, and dimethyl phosphatidylethanolamine may be determined simultaneously. An additional method for radial HPTLC determination of saturated phosphatidylcholine is described. We report...

BACKGROUND Over exploitation of many traditional medicinal plants like Myrica esculenta has become a threat and in the near future, many medicinal plants may be unavailable for use of industry. OBJECTIVE Present study outlines the concept of plant part substitution. Stem bark and small branches of M. esculenta are compared on the basis of physicochemical analysis, phytochemical analysis, tota...

A simple high performance thin layer chromatographic (HPTLC) method has been developed for the simultaneous determination of the pharmacologically important active combines the separation and quantification of the analytes on silica gel 60 GF 254 HPTLC plates with visualization under UV and scanning at 540nm. The method is rapid, simple and precise.

The identification of drugs by the combination of two-dimensional HPTLC and remission spectrometry is described. The discriminating power and information content of this combination are calculated and compared with the corresponding values of GC/MS. Advantages and disadvantages of HPTLC and applications to some drugs are mentioned.