Naturally occurring transposable element (TE) insertions that disrupt Drosophila promoters are correlated with modified promoter function and are posited to play a significant role in regulatory evolution, but their phenotypes have not been established directly. To establish the functional consequences of these TE insertions, we created constructs with either TE-bearing or TE-lacking hsp70 prom...

The role of heat shock protein 70 (Hsp70) in virus replication has been discussed for many viruses. The known suppressive role of Hsp70 in influenza virus replication is based on studies conducted in cells with various Hsp70 expression levels. In this study, we determined the role of Hsp70 in influenza virus replication in HeLa and HEK293T cells, which express Hsp70 constitutively. Co-immunopre...

We have examined the expression of the heat shock protein (hsp7O) gene in human cells. The transcription of the hsp7O gene and accumulation of cytoplasmic hsp70 mRNA is induced by serum stimulation. Populations of HeLa cells and human embryonic kidney cells (cell line 293) were serum starved. Upon serum stimulation, the level of hsp70 mRNA transiently increases between 12 and 18 hr to a 10-fold...

We have examined the transcriptional regulation of the 70-kDa (70K) heat shock gene family following infection of human and monkey cells with four different DNA viruses: adenovirus type 5 (Ad5), herpes simplex virus type 1 (HSV-1), simian virus 40, and vaccinia virus. Our results indicate that induction of these genes is not a general response to the stress of viral infection but is instead a h...

Drosophila melanogaster collected in sub-equatorial Africa in the 1970s are remarkably tolerant of sustained laboratory culture above 30 degrees C and of acute exposure to much warmer temperatures. Inducible thermotolerance of high temperatures, which in Drosophila melanogaster is due in part to the inducible molecular chaperone Hsp70, is only modest in this strain. Expression of Hsp70 protein ...

The development of transgenic reporter mice and advances in in vivo optical imaging have created unique opportunities to assess and analyze biological responses to thermal therapy directly in living tissues. Reporter mice incorporating the regulatory regions from the genes encoding the 70 kDa heat-shock proteins (Hsp70) and firefly luciferase (luc) as reporter genes can be used to non-invasivel...

Exposure of pulmonary artery endothelial cells (PAECs) to hyperoxia results in a compromise in endothelial monolayer integrity, an increase in caspase-3 activity, and nuclear translocation of apoptosis-inducing factor (AIF), a marker of caspase-independent apoptosis. In an endeavor to identify proteins involved in hyperoxic endothelial injury, we found that the protein expression of heat-shock ...

The effect of live yeast Rhodoturula glutinis was evaluated on juvenile Oreochromis niloticus cultivated in a Biofloc system. Growth performance and the expression innate response relevant oxidative stress genes were after 12-week feeding trial. Three experimental treatments conventional tilapia culture (control), (BFT), with addition R. 1×106 CFU g-1 (BFT+Rg), four replicates per treatment. In...

The birth rate of embryos produced in vitro (IVF) is still lower than that vivo. Three major steps for the success IVF technique are maturation immature oocytes, fertilization matured and culture resulting embryos. Studying mRNA expression early embryonic development stages important can help to assess embryo quality optimize production protocols vitro. current study aimed determine levels deve...

Transcriptional regulation of the human hsp70 gene in response to heat shock and other forms of physiological stress occurs through the activation of heat shock transcription factor (HSF). Exposure of cells to a heat shock temperature of 42 degrees C results in transient activation of HSF; its DNA-binding activity increases rapidly, plateaus, and attenuates, during which the intracellular level...