AIMS To assess the validity and practicality of real time polymerase chain reaction (PCR) for human papillomavirus (HPV) testing in combination with liquid based cytology samples for cervical screening. METHODS Real time PCR using consensus (GPS+/6+) and type specific primers was developed to detect genital HPV types. This provides rapid, efficient amplification followed by denaturation of th...

The relationship between human papillomavirus (HPV) DNA in the genital mucosa and serum IgG to HPV-16, -18, and -6 was studied in a cohort of 588 college women. Among women with incident HPV infections, 59.5%, 54.1%, and 68.8% seroconverted for HPV-16, -18, or -6, respectively, within 18 months of detecting the corresponding HPV DNA. Transient HPV DNA was associated with a failure to seroconver...

Human papillomavirus, type 16 (HPV-16) and type 18 (HPV-1 8) are known to play a role in the development of neoplastic disorders of the urogenital organs. The presence of HPV-1 6 and HPV18 in prostatic tissue with benign hyperplasia has been a matter of controversy. Some investigators have reported a high prevalence of HPV-16 DNA and a low prevalence of HPV-18 DNA in prostatic tissue,'-3 while ...

BACKGROUND Human papillomavirus (HPV) seroprevalence data have not previously been reported for different geographical regions of China. This study investigated the cross-sectional seroprevalence of antibodies to HPV 6, 11, 16, and 18 virus-like particles in Chinese women. METHODS Population-based samples of women were enrolled from 2006 to 2007 in 3 rural and 2 urban areas of China. Each con...

OBJECTIVES Cervical cancer is the most common cause of cancer-related deaths among South African women. Viral types associated with cervical cancer may differ not only between countries and regions, but possibly also between human immunodeficiency virus (HIV)-infected and noninfected women. METHODS In a population with high HIV prevalence, human papillomavirus (HPV)-type infections detected w...

BACKGROUND External genital warts (EGW) are relatively common sexually transmitted diseases. In the majority of cases, low-risk human papilomaviruses (HPV), such as HPV-6 and HPV-11, are responsible but, high-risk types may also be detected and this has a bearing on vaccines for cervical cancer prevention. In this study the incidence of the high-risk HPV types 16, 18, 33 and 52 in EGWs of femal...

AIMS To determine which type of human papillomavirus (HPV) is associated with cervical adenocarcinoma and whether the virus was integrated or episomal in two continents. METHODS Biopsy specimens from the UK (n = 16) and South Africa (n = 22) were analysed by non-isotopic in situ hybridisation (NISH) for HPV types 6, 11, 16, 18, 31, 33, and 35 on archival biopsy specimens using digoxigenin lab...