background: spermatozoa cryopreservation is used for the management of infertility and some other medical conditions. the routinely applied cryopreservation technique depends on permeating cryoprotectants, whose toxic effects have raised the attention towards permeating cryoprotectants-free vitrification technique. objective: to compare between the application of slow cryopreservation and vitri...

Common carp [Cyprinus carpio (C. carpio)] is an economically important fish species cultured mostly in Asia and Europe. Global production of farmed common carp was about 6.14% (3 172 488 tonnes) of the total world aquaculture production[1]. Artificial insemination can be applied to increase the production to meet this demand due to exploitation of natural stocks. Cryopreservation is a valuable ...

This study tested different concentrations of egg yolk in canine freezing extender void of glycerol, a commonly used cryoprotectant, by examining the motility and morphology throughout the freezing process: initial (baseline after extender added), post-cool (after three hours at 5°C) and post-thaw (after freezing.) Initial values of pH, osmolarity, motility and morphology were obtained for comp...

Freeze-storage of epididymal sperm is an important technique for the preservation of gametes in animals, including those becoming extinct. We froze canine sperm recovered from the cauda epididymis and investigated the fertility. The qualities of sperm from the cauda epididymis before freezing were: mean sperm motility, 89.4 +/- 1.6 (SE) %; sperm viability, 89.1 +/- 1.1%; and these were signific...

the aim of this study was to compare the effects of using different levels of royal jelly in semen extender on viatal characteristics of goat sperm after freezing. semen was collected from four adult bucks twice a week and samples pooled together. sperm samples (six repeats) containing royal jelly in four levels (0.5, 1, 1.5 and 2%) had been frozen. after semen samples freezing – were thawed an...

A sperm cryopreservation protocol requiring dimethylacetamide (DMA, 6%) as a cryoprotectant was optimized via assays involving different prefreezing equilibration times (1, 10, 30, 60, and 120 min at 5°C) and different freezing rates achieved by the following: 1) using nitrogen vapor to reduce the temperature from 5°C to -85°C at 10°C/min (slow freezing rate); 2) using a biological freezer unit...

Boar sperm viability post-thaw differs depending on the ejaculate fraction used, with spermatozoa present in the first 10 mL of the sperm-rich fraction (SRF) (portion 1, P1, sperm-peak portion) displaying the best cryosurvival in vitro compared with that of spermatozoa from the rest of the ejaculate (portion 2 of the SRF plus the post-spermatic fraction), even when using simplified freezing rou...

Cryopreservation induces injuries to fish spermatozoa that in turn affect sperm quality in terms of fertilization ability, motility, DNA and protein integrity and larval survival. To reduce the loss of sperm quality due to freezing-thawing, it is necessary to improve these procedures. In the present study we investigated the ability of two antifreeze proteins (AFPI and AFPIII) to reduce the los...

reactive oxygen species (ros) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. curcumin is known as an effective antioxidant against oxidative stress. the aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and dna integrity of rat spermatozoa during semen freeze-tha...

Cryopreservation of spermatozoa plays a significant role in reproductive medicine and fertility preservation. Chicken egg yolk is used as an extender in cryopreservation of human spermatozoa using glycerol egg yolk citrate (GEYC) buffered medium. Even though 50% survival of spermatozoa is generally achieved with this method, the risk of high levels of endotoxins and transmission pathogens from ...