There is an association between sedentary behavior and the risk of colorectal cancer (CRC), but the underlying mechanism is unclear. CRC is characterized by the changes in the expression levels of biomarkers, including voltage-gated proton channel Hv1, matrix-remodeling associated 5 (MXRA5), DEK (inducing positive supercoils into circular DNA) and protein inhibitor of activated signal transduce...

Gastroenterology Medicine & Research An Immunosuppression Hidden Malignancy: Case Report of Unexpected Clostridium Septicum Infection and Its Fatal Consequence Grade M1*, Welk E1, Sostmann H1, Hammerstein HV1, McAulay CH3 Bostelmann R2 1Department Gastroenterology, Infectious Diseases General Internal Christian Hospital Quakenbrück, Germany 2Department Neurosurgery Spine Surgery, 3Department Ra...

Voltage-clamp fluorometry (VCF) supplies information about the gating transitions of voltage-gated proteins. Changes in fluorescence intensity dye attached to a part protein that undergoes conformational rearrangement upon alteration membrane potential by electrodes constitute signal. The VCF signal is generated quenching and dequenching as traverses various local environments. Here we studied ...

Proton channels have evolved to provide a pH regulatory mechanism, affording the extrusion of protons from the cytoplasm at all membrane potentials. Previous evidence has suggested that channel-mediated acid extrusion could significantly change the local concentration of protons in the vicinity of the channel. In this work, we directly measure the proton depletion caused by activation of Hv1 pr...