Glycogen branching enzymes (GBEs; 1,4-α-glucan enzyme; E.C. 2.4.1.18) have so far been described to be capable of both α-1,6-transglycosylation (branching) and α-1,4-hydrolytic activity. The aim the present study was elucidate mode action three distantly related GBEs from glycoside hydrolase family 13 by in depth analysis activity on a well-defined substrate. For this purpose, R. marinus (RmGBE...

Zinc is an essential element required for the function of more than 300 enzymes spanning all classes. Despite years of dedicated study, questions regarding the connections between primary and secondary metal ligands and protein structure and function remain unanswered, despite numerous mechanistic, structural, biochemical, and synthetic model studies. Protein design is a powerful strategy for r...

What is the composition of snake venom?  Snake venom is a cocktail of several different proteins and enzymes  Protein constitute 90% of dry weight of venoms o Proteins include Lethal Polypeptides and Hydrolytic enzymes (Proteases, Nucleases, Peptidases, Lipases, etc) that can either enhance and/or contribute to the toxic effect of the venom o Enzymes cause both local and systemic destruction ...

Background: Candida species is a normal commensal flora of human body inhabiting the skin, mucous membrane and gastro intestinal tract but may be associated with superficial deep-seated fungal infections. The switch from to potent pathogen, facilitated by various extracellular hydrolytic enzymes. aim this study was estimate phospholipase, proteinase, haemolysin esterase activity determine antif...