Viral load measurements are an essential tool for the long-term clinical care of hum an immunodeficiency virus (HIV)-positive individuals. The gold standards in viral load instrumentation, however, are still too limited by their size, cost, and sophisticated operation for these measurements to be ubiquitous in remote settings with poor healthcare infrastructure, including parts of the world tha...

Diagnostic methods for detecting and quantifying HIV RNA have been improving, but efficient methods for point-of-care analysis are still needed, particularly for applications in resource-limited settings. Detection based on reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is particularly useful for this, because when combined with fluorescence-based DNA detection, RT-LAMP ...

A sensitive duplex-immunocapture-RT-PCR (D-IC-RT-PCR) technique was developed for detection and discrimination of taxonomically distinct Sugarcane streak mosaic virus (SCSMV) and Sugarcane mosaic virus (SCMV) that naturally infect sugarcane. D-IC-RT-PCR was performed using polyclonal antisera for capture of virions. Oligo 5'-d(T)18(AGC)-3' as a common reverse primer for both viruses and virus s...

Tobacco mosaic virus (TMV) causes significant losses in many economically important crops. Contaminated soils may play roles as reservoirs and sources of transmission for TMV. In this study we report the development of an immunocapture real-time RT-PCR (IC-real-time RT-PCR) assay for direct detection of TMV in soils without RNA isolation. A series of TMV infected leaf sap dilutions of 1:101, 1:...

The objective of this study is to compare and assess the effectiveness real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), selective agar plate method for detection Salmonella spp. Listeria monocytogenes in ready-to-eat (RTE) foods. In RTE foods, performance three methods (RT-PCR [SureTectTM kit PowerChekTM kit], LAMP [3M MDS], agar) were similar at 0-10...

Introducción: el coronavirus SARS-CoV-2, causante de la enfermedad COVID-19 se ha convertido en un problema salud pública mundial que requiere implemen-tación pruebas diagnóstico rápidas y sensibles. Objetivo: evaluar comparar límite detección del método LAMP con respecto al estándar aplicar RT-LAMP para SARS-CoV-2 muestras clínicas pacientes colombianos. Métodos: realizó estudio descriptivo tr...

Real-time RT-PCR is used as a gold standard method for the diagnosis of COVID-19. Since real-time RT PCR nucleic acid-based, it highly sensitive and specific test. However, this test takes 4–8 h to generate results and, in emergency settings, delay may prove fatal certain patients. The frequent surge COVID cases increases patient load settings. Thus, acid-based rapid POC required that can quick...

The fragment of the membrane protein M gene with high conservation and specificity of porcine reproductive and respiratory syndrome virus (PRRSV) was chosen to be the target region, according to which six special primers were designed successfully. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was applied to detect the PRRSV by incubation at 65° for only 45 min with the...