a new hybrid nanomaterial was developed by immobilization of phosphomolybdate anions on the surface of modified magnetite nanoparticles with quaternary phosphonium cations. silica coated magnetic nanoparticles supported phosphonium cations, ph3p+-scmnps, were prepared by covalent attachment of chloropropylsilyl groups on the surface of silica coated magnetite nanoparticles and next reaction wit...

objective: organophosphorus (ops) compounds are widely used in many pesticides, insecticides and chemical nerve agents. these compounds are hazardous for humans and the environment. organophosphate hydrolase (oph) is a homodimeric protein initially isolated from pseudomonas diminuta mg and flavobacterium species. this enzyme is able to degrade a broad spectrum of toxic ops compounds. using immo...

we expressed and purified a recombinant p. pyralis luciferase with n-terminal his-tags. the silanized ni or cu-loaded magnetic particles were prepared and used to assemble the his-tagged p. pyralis luciferase. this enzyme immobilized on functionalized magnetic nanoparticles (mnps) via electrostatic interactions of his-tag with ni2+/cu2+ ions on the surface of mnps using simple one step method. ...

lateral flow assay, a simple and rapid test to detect various agents; have found many applications in different fields. one of the most important issues in the construction of a lateral flow assay is the increasing of its sensitivity and efficiency by processing of analytical layer. this study, aimed to investigate the performance of, nellulose, nitrocellulose, nylon and pvd as different analyt...

background sulfur oxides released from the burning of oil causes severe environmental pollution. the sulfur can be removed via the 4s pathway in biodesulfurization (bds). immobilization approaches have been developed to prevent cell contamination of oil during the bds process. objectives the encapsulation of rhodococcus erythropolis r1 in calcium alginate beads was studied in order to enhance c...

background there is controversy regarding routine prophylaxis for deep vein thrombosis (dvt) in patients treated via a short leg cast or splint following lower extremity trauma. results there were 95 patients with an average age of 38 ± 13.7 (77.9% males); 46 patients had at least one risk factor for dvt. the d-dimer test was positive in 21(22.1%) patients. dvt was confirmed by due in 3 patient...

background: collagen, the most abundant protein in the human body, and as an extracellular matrix protein, has an important role in the fiber formation. this feature of the collagen renders  establishment of the structural skeleton in tissues. regarding specific features associated with the collagen, such as, formation of the porous structure, permeability and hydrophilicity, it can also be use...

purpose: the present study aimed to investigate the effect of immobilization stress on the induction of diabetes mellitus in rats. materials and methods: in this experimental study 30 mature male rats with an average weight of 200-220 gr were randomly divided into three groups of ten each. group i served as the control while the experiment groups were group ii (10 days) and groups iii (30 days)...

the protease enzyme breaks down protein into amino acids as its constructors. protease immobilization onto appropriate support materials plays an essential role in various fields of technology including the food and detergent industries. accordingly, improvement of protease immobilization has been highly regarded due to its applications in bio catalysis. alginate and chitosan are natural polysa...