This report describes the detailed procedures for Western blot analysis using fluorescent antibodies. After electrophoresis and subsequent electroblotting, the fluorescent-labeled antibodies were visible upon ultraviolet illumination of the polyvinylidene fluoride (PVDF) membranes and could then be photographed to give an accurate record of the blots. Fluorescent labeling allows for photographi...

The objectives of this study were to compare the sensitivities and reliabilities of direct agglutination test (DAT), indirect immunofluorescent antibody test (IFAT), polymerase chain reaction (PCR) and bone marrow aspiration for the diagnosis of Mediterranean visceral leishmaniasis (MVL) using blood samples. Blood samples were collected from 67 confirmed MVL patients and 47 patients with other ...

Direct detection of fecal coliforms as indicators of fecal pollution may assess water quality more accurately than culture methods. An irnmunochemical technique involving the use of specific antibodies was applied in addition to plate counts to evaluate Escherichia coliin seawater samples from different coastal areas in southern Italy. The immunofluorescent method showed high specificity for en...

We found high correlations when the values for immunoglobulins G, A, and M, obtained from the analysis of a group of 50 or more human sera by a measured-time radial immunodiffusion method, were compared to values found by two new commercial immunofluorescent methods. The Fiax and the Immuno-Fluor systems make use of fluorescently labeled antibodies and solid-phase immunoadsorbents and off...

Monoclonal antibodies have become increasingly important in the diagnosis and classification of neoplastic blood disorders. Immunofluorescent labelling of cell surface antigens has been the conventional method used for this purpose. However, this method of antigen detection presents a number of drawbacks when used in a routine hospital haematology laboratory: namely that blood or bone marrow sa...