We predicted that a significant source of background labeling after in situ hybridization (ISH) using 35 S-labeled probes is attributable to a chemical reaction between the phosphorothioate moiety of the probe [O 3 P 5 S] and disulfides in tissue. These covalent bonds would immobilize probe in the tissue, thereby increasing background labeling. On the basis of this view, we have explored the us...

PURPOSE Laser-assisted in situ keratomileus (LASIK) creates a permanent flap that remains non-attached to the underlying laser-modified stroma. This lack of permanent adhesion is a liability. To immobilize a corneal flap, a protocol using fibrinogen (FIB), riboflavin (RF), and ultraviolet (UVA) light (FIB+RF+UVA) was devised to re-adhere the flap to the stroma. METHODS A model flap was create...

Microorganisms are intimately involved in metal biogeochemistry with a variety of processes determining mobility, and therefore, bioavailability. The balance between mobilization and immobilization varies depending on the organisms involved, their environment and physicochemical conditions. Metal mobilization can arise from a variety of leaching mechanisms, complexation by metabolites and sider...

The invention deals with a novel method for immobilization enzymes on solid surfaces such as metallic and graphite ones, which overcomes the disadvantages of standard current methods. Technology The immobilization technique is accomplished by doping the surface with a blend of the enzymes to be fixed, glutaraldehyde and nafion-ion exchanger, as protective additive. Therefore, the above techniqu...

OBJECTIVES Corticotropin-releasing factor (CRF) plays a prominent role in mediating the effect of stressors on the hypothalamic-pituitary-adrenal axis. In this study, we examined the effects of chronic administration of second-generation antipsychotic drug ziprasidone on CRF mRNA expression in the hypothalamic paraventricular nucleus (PVN) of rats with or without immobilization stress. METHOD...

Nanopatterning of biomolecules on functionalized surfaces offers an excellent route for ultrasensitive protein immobilization, for interaction measurements, and for the fabrication of devices such as protein nanoarrays. An improved understanding of the physics and chemistry underlying the device properties and the recognition process is necessary for performance optimization. This is especially...

In this work, the effect of immobilization of bread yeast (Saccharomyces cerevisiae) by sol-gel technique combined with functionalized nanoporous silica was investigated in different weight ratios of silica containing materials (TMOS: LUS-1). The activities of immobilized yeast in days after immobilization were examined. The results showed immobilization maintain the yeast life for a longer tim...

In this work, the effect of immobilization of bread yeast (Saccharomyces cerevisiae) by sol-gel technique combined with functionalized nanoporous silica was investigated in different weight ratios of silica containing materials (TMOS: LUS-1). The activities of immobilized yeast in days after immobilization were examined. The results showed immobilization maintain the yeast life for a longer tim...

Case Description- Five lions (one female and 4 male) were presented for diagnostic andsurgical treatment (wound management, castration and radiography). Immobilization oranesthesia was necessary to complete the procedure.Treatment and Outcome - Immobilization was induced using intramuscular injection ofvarious drug combinations (xylazine, diazepam or midazolam in combination withketamine). Inha...

Over the past years, DNA biosensors have been developed to analyze DNA interaction and damage that have important applications in biotechnological researches. The immobilization of DNA onto a substrate is one key step for construction of DNA electrochemical biosensors. In this report, a direct approach has been described for immobilization of single strand DNA onto carboxylic acid-functionalize...