A complete protocol for micropropagation of Musa sapientum using shoot meristems was developed. Multiple shoots were induced in vitro from shoot meristems. Murashige and Skoog’s medium supplemented with BAP and NAA (3.0 + 0.2 mg/l, respectively) was found to be most suitable combination. Further multiplication of shoots required habituation of cultures up to 3 passages of 21 days each on the sa...

The effect of sucrose concentration on the micropropagation axillary shoots willow was investigated. following factors were examined: culture system (semisolid medium in glass jars versus liquid temporary immersion bioreactors), type explant (apical and basal sections), frequency immersion, CO2 enrichment. Shoots leaf growth significantly higher RITA® bioreactors than for all treatments. Apical...

plant tissue culture is an alternative method of commercial propagation and is being used widely for the commercial propagation of a large number of plant species, including many medicinal plants. somatic embryogenesis is a process by which asexual or somatic cells are induced to form embryos in culture. somatic embryogenesis is a multi-step regeneration process starting with formation of pro-e...

background & aim: clematis is an ornamental and medicinal plant that is primarily planted as an ornamental plant. clematis orientalis is native to asia and central europe, used as an antiseptic and refrigerant. the propagation of clematis is very important. in this study, the effects of plant growth regulators on rooting and growth of the plants in vitro culture were examined. experimental:...

Shoot tips and nodal segment explants to Rauwolfia serpentina when cultured on MS medium containing varying concentration of Benzyl adenine purine (BAP) and Benzyl adenine purine in combination with Indole butyric acid (IBA) produced multiple shoots. Maximum multiple shoots (85.6%) were found in static MS medium supplemented with 5.0 mg/ 1 BAP and 0.5 mg / 1 IBA along with 2.5% sucrose and 0.85...

Micropropagation is an effective way to preserve the gene pool of threatened plants. This study devoted mathematical modeling nutrient media and effect mT (meta-topoline) on multiplication shoots Hedysarum grandiflorum, Hyssopus cretaceus, Matthiola fragrans in vitro comparison with benzylaminopurine (BAP) kinetin (KT). Initiation was performed MS medium 0.5 mg/L BAP. For multiplication, MS, B5...

The Vaccinium genus contains several valuable fruit and ornamental species, among others: highbush blueberry (Vaccinium × corymbosum L.), cranberry (Vaccinium macrocarpon Ait.), and lingonberry (Vaccinium vitis-idaea L.). In some most popular and valuable cultivars, the conventional propagation methods, exploiting hard or soft wood cuttings, are inefficient. The demand for nursery plants could ...

An in vitro micropropagation protocol was developed for Capsicum chinense Jacq. popularly known as habenaro pepper which is one of the world’s hottest chilli and it is a traditional food item of the Naga people. Nagaland is believed to be the original home for the chilli pepper. The Guinness book of World record has awarded this chilli as hottest one as it contains 1,041,427 Scoville Heat units...

Scented rose species are very important in the genus Rosa due to their use in highly-prized essential oil production and as garden roses. The fragrance of the rose flower (attar) captured by extraction is one of the most valuable flavor and fragrance products. A comprehensive study of the papers published in the field of in vitro culture and biotechnology of these species, particularly Damask r...