Results of Chagas' disease diagnosis show disagreement. The aim of this study was to compare commercial tests for Chagas' disease serodiagnosis in southern Brazil. A total of 161 samples were evaluated. Three enzyme-linked immunosorbent assays, one indirect hemagglutination and one indirect immunofluorescence were assessed. Trypomastigote excreted-secreted antigen-blot was a confirmatory method...

An antiserum raised against whole cells of Thiobacillus ferrooxidans was allowed to react with a variety of acidophilic and nonacidophilic bacteria in an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay. Both experiments demonstrated that the antiserum was specific at the species level. This preparation was used to evaluate the role of T. ferrooxidans in the microbial ...

We examined 138 Escherichia coli strains isolated from cultures of blood specimens from urosepsis patients (n = 78) and nonurosepsis patients (n = 30) and from fecal specimens of a healthy control group (n = 30) for P fimbriation with a mannose-resistant hemagglutination test, an indirect immunofluorescence assay, and a new commercial particle agglutination test (PF TEST; Orion Diagnostica, Esp...

background: the humbug gene is a truncated isoform of aspartyl β-hydroxylase (asph) gene that is overexpressed in many human malignancies. in recent years, since humbug has received increasing attention, it is considered as a potential therapeutic molecular target. therefore, it is necessary for preparing humbug protein and its monoclonal antibody to investigate its structure and function. meth...

IMPORTANCE Pemphigus vulgaris (PV) is a disease that features blistering of the skin and mucous membranes caused by autoantibodies directed against desmoglein 3 (Dsg3) and/or desmoglein 1(Dsg1). Indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA) are 2 methods that are widely used to measure Dsg3 orDsg1 antibody titers in PV. Although the titers of these autoantibodi...

The AIDS epidemic is spreading rapidly among women worldwide, offering increasing opportunities for vertical transmission of HIV. In Brazil, the prevalence of HIV infection among pregnant women is less than 1%. Therefore, the positive predictive value of an HIV EIA test tends to be lower than the more frequent indeterminate Western blot result. Pregnant women receiving antenatal care, from 2000...