In man-Chinese hamster somatic cell hybrids the segregation of the loci for 27 human enzyme markers and the species-specific surface antigens, including the HL-A histocompatibility antigens, was studied. The results show a synteny of the human loci for phosphoglucomutase 3, cytoplasmic malic enzyme, tetrameric indophenol oxidase, and HL-A. Furthermore, evidence is presented that the loci for th...

In an earlier note, evidence was presented for the occurrence of a new type of chloroplast-catalyzed photosynthetic phosphorylation called “oxidative photophosphorylation” (I). When spinach chloroplasts are illuminated in the presence of orthophosphate, adenosine diphosphate, and a dye such as trichlorophenol indophenol, adenosine triphosphate is generated provided oxygen is available, but not ...

I describe a senstive colorimetric micromethod in which a pH 11.0 carbonate buffer is used to induce acetaminophen to react with Folin-Ciocalteau reagent at room temperature. These reagents and a simple solvent extraction favor formation of a stable indophenol dye with acetaminophen, giving this procedure a good degree of specificity. Results correlate well with those by liquid-chromatographic ...

A technique has been developed for the rapid and simple measurement of ribulose 1,5-bisphosphate from isolated spinach chloroplasts. The endogenous ribulose bisphosphate was detected enzymically using (14)CO(2) and ribulose bisphosphate carboxylase/oxygenase released from the chloroplasts. Ribulose 5-phosphate kinase was inhibited with 0.4 to 0.6 millimolar 2,6-dichlorophenol-indophenol and 4 m...

oxidase 3 . Moreover, methylene blue was shown to be a strong inhibitor of the overall reaction. Oneelectron acceptors are also ineffective with the Dspecific enzyme; however, methylene blue and 2.6dichlorophenol-indophenol are able to reoxidize the reduced D-6-hydroxynicotine oxidase. In the presence of these dyes the rates of the overall process are higher than those with oxygen. Anaerobic ti...

A simple, rapid, and specific spectrophotometric method was developed for the determination of paracetamol in pure form drug formulations. The depended on hydrolysis to p-aminophenol, which, oxidized under effect dissolved oxygen benzoquinoneimine. In alkaline medium, benzoquinoneimine reacts with Methyldopa produce a high stabile indophenol dye. absorbance measured at 580 nm, molar absorptivit...

Widely varying values for the molar extinction coefficient of 2.6-dichlorophenoi indophenol have been reported by various workers 1-9. A possible source of this variation may be the different samples of DCIP used, due either ~o the presence of impurities or to the presence of mixtures of the anhydrous and hydrated forms of DCIP sodium salt. However. failure to realise that DCIP is a weak acid w...

2,6-Diclhloroplleniolinidoplheol (D LP1P) is one of tlle mainy comnmloiinly) used electroni acceptors for the light-induced reactions in chloroplasts (2). Until recently, it has generally been assumed that it accepts electrons from the short-wavelengtlh pigment system 2 (PS2). Since the finding that indophenol reduction can be coupled with phlotophosphorylation [see review by Vernon and Avron (...

On the open-loop and feedback processes that underlie the formation 1 of trajectories during visual and nonvisual locomotion in humans 2 3 4 Quang-Cuong Pham, Halim Hicheur 5 6 1 Laboratoire de Physiologie de la Perception et de l'Action, Collège de France CNRS UMR 7152, 7 11 place Marcelin Berthelot, 75005 Paris, France 8 2, present address Hertie Institute for Clinical Brain Research, Departm...