Bacterial RecA promotes the development and transmission of antibiotic resistance genes by self-assembling into an ATP-hydrolyzing filamentous homopolymer on single-stranded DNA. We report the design of a 29mer peptide based on the RecA N-terminal domain involved in intermonomer contact that inhibits RecA filament assembly with an IC50 of 3 microM.

Three new meroterpenoids, named austalides S-U (1-3), were isolated from the culture of a sponge-derived fungus Aspergillus aureolatus HDN14-107, together with eleven known austalides derivates (4-14). Their structures, including absolute configurations, were assigned on the basis of NMR, MS data, and TDDFT ECD calculations. Compound 1 is the first case of austalides with the terpene ring fused...

A novel series of 2-substituted-4,6-diarylpyrimidines 6a-6t has been synthesized, characterized by ¹H-NMR, 13C-NMR and HRMS, and screened for in vitro α-glucosidase inhibitory activity. The majority of the screened compounds possessed significant α-glucosidase inhibitory activity with IC50 values ranging from 19.6 ± 0.21 to 38.9 ± 0.35 μM, which is more potent than the positive control α-glucos...

A series of novel N-phenylbenzamide and N-phenylacetophenone compounds were synthesized and evaluated for their antiviral activity against HCV and EV71 (strain SZ-98). The biological results showed that three compounds (23, 25 and 41) exhibited considerable anti-HCV activity (IC50=0.57-7.12 μmol/L) and several compounds (23, 28, 29, 30, 31 and 42) displayed potent activity against EV71 with the...

Three new sesquiterpenoids (1-3) and one new sesquiterpenoid derivative (4), along with three known sesquiterpenoids (5-7), were isolated from the 95% ethanolic extract of Chinese eaglewood [Aquilaria sinensis (Lour.) Gilg]. The structures of these compounds were elucidated through extensive analysis of spectroscopic data including IR, NMR, HRESIMS, and X-ray diffraction experiments. In additio...

In oxygen-aerated non-growing cells a subinhibitory ethanol concentration (1 G/L) causes an H /K exchange. An inhibitory ethanol concentration (30 G/L) slows down acidification but + + has no effect on its extent. The transmembrane pH depends directly on the ethanol level in the medium and is not lowered at high ethanol concentrations. Changes in membrane potential induced by ethanol are also c...

the purpose of this study was to determine the prevalence of anti-microbial resistance in intubated patients in intensive care unit (icu) of bahonar hospital in kerman province, iran during the year 2008. tracheal samples were obtained from 111 intubated patients in the icu by broncoalveolar lavage method. amikacin, ceftazidim and imipenem were used to evaluate antibiotic susceptibility. for de...

materials and methods one hundred ssis specimens were obtained by needle aspiration from purulent material in depth of infected site. these specimens were cultured and incubated in both aerobic and anaerobic condition. for detection of antibiotic susceptibility pattern in aerobic and anaerobic bacteria, we used disk diffusion, agar dilution, and e-test methods. results a total of 194 bacterial ...