Gram-negative Pseudomonas spp. particularly P. aeruginosa is an important pathogen in hospitalized patients, contributing to the morbidity and mortality due its multiple resistance mechanisms. The present study was undertaken determine characteristics of isolated from pus patients admitted a local diagnostic center, Dhaka, Bangladesh. A total 14 clinical isolates were obtained. Detailed biochem...

We analyzed the role of integrons in the dissemination of antibiotic resistance in a recent multiresistant clinical isolate, Serratia marcescens SCH88050909 (SCH909). This isolate harbors three integrons, all on a 60-kb conjugative plasmid. By PCR, hybridization, and sequencing analyses, we found that integron 1 has the dfrA1 and ant(3")-Ia cassettes. The first cassette in integron 2 contains t...

Objectives: Dissemination of antimicrobial resistance (AMR) is a global issue that requires the adoption of a "One-Health" approach promoting integration of human and animal health. Besides culture-dependent techniques frequently used for AMR surveillance, cultivation-independent methods can give additional insights into the diversity and reservoir of AMR genetic determinants. Integrons are mol...

Integrons are non-mobile bacterial genetic elements that carry different cassettes conferring antibiotic resistance. Cassettes can excise or integrate by action of an integron-encoded integrase, enabling bacteria to face environmental challenges. In this work, the functionality and dynamics of two integrons carrying the same cassette arrangement (dfrA12-orfF-aadA2), but located on plasmid or ch...

OBJECTIVES Integrons are bacterial genetic elements that can capture and express genes contained in mobile cassettes. Integrons have been described worldwide in Gram-negative bacteria and are a marker of antibiotic resistance. We developed a specific and sensitive Taqman probe-based real-time PCR method with three different primer-probe pairs for simultaneous detection of the three main classes...

This study was aimed to find out that broiler chicken farms have problems with antibiotic resistance Citrobacter freundii and determined the prevalence class 1 integron encoding gene. Multidrug collected from among one hundred sixty cloacal swab samples 32 in Blitar for 3 months. The method of bacterial inoculation used MacConkey agar biochemical test conducted by IMViC TSIA test. sensitivity p...

background: infection with non-typhoid salmonella (nts) is one of the most important health problems all over the world. antimicrobial drug resistance is increasing among salmonella infantis species. objectives: the aim of this study was to investigate the frequency of presence of class 1 integrons in s. infantis species as well as its association with drug resistance. materials and methods: th...

background: integrons are the major reasons of multidrug resistance (mdr) among enteropathogenic bacteria. occurrence of horizontal gene transfer between integron-carrying microorganisms and other enteric bacteria may increase the rate of emergence of integron-associated antibiotic resistance. objectives: the objective of this study was to investigate class 1 integrons among members of enteropa...

BACKGROUND Emergence and global dissemination of multiresistant strains of enteric pathogens is a very concerning problem from both epidemiological and Public Health points of view. Shigella sonnei is the serogroup of Shigella most frequently responsible for sporadic and epidemic enteritis in developed countries. The dissemination is associated most often to human to human transmission, but foo...

Lateral gene transfer (LGT) impacts on the evolution of prokaryotes in both the short and long-term. The short-term impacts of mobilized genes are a concern to humans since LGT explains the global rise of multi drug resistant pathogens seen in the past 70 years. However, LGT has been a feature of prokaryotes from the earliest days of their existence and the concept of a bifurcating tree of life...