The most commonly used assay to quantitate the response of peripheral T-cells upon stimulation with growth factors is determination of incorporated [3H]TdR. We compared this test to three other methods: 1. direct counting of cells with a Coulter type counter as reference assay, 2. a colorimetric assay using the tetrazolium dye 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium (MTT), which i...

background and objective: preterm birth occurs in 8% to 11% of all pregnancies and is responsible for 75% to 80% of all neonatal deaths. cytokines may be involved in the etiology of preterm birth through their stimulation of prostaglandin synthesis. cytokines may be released intocervicovaginal fluid during the breakdown of the chorio-decidual adhesion or from an inflammatory reaction in the sam...

To analyze the levels of inflammatory factor and safety in treating psoriasis with apremilast. We selected 200 cases patients under-treated our hospital from January 2021 to 2022, divided them into control group experimental at random, 100 cases, respectively. Control received normal treatment, while treated Compare both groups on factors, skin lesions adverse drug reactions. Before had no stat...

Impaired immune reconstitution has moved to the forefront of clinical problems limiting progress in allogeneic bone marrow transplantation (BMT). The identification of therapies that can enhance immune reconstitution by increasing thymopoiesis is critical to solving this problem. Interleukin 7 (IL-7) is the most potent thymopoietic cytokine identified thus far. To study the effects of IL-7 on i...

The ability of interleukin-7 (IL-7) to stimulate murine thymocyte proliferation was investigated. IL-7, either alone or in concert with lectin, induced proliferation of adult thymocytes as well as day 13 fetal and adult CD4-/CD8-thymocytes. The IL-7-induced proliferative response of unfractionated thymocytes could not be inhibited by antibodies to IL-2, or IL-4, IL-6, or the IL-2 receptor. In a...

In contrast to adults, the murine neonatal CD4+ compartment contains a high frequency of recent thymic emigrants (RTEs). However, the functional capabilities of these cells in neonates are relatively unknown. Moreover, it has not been determined whether RTEs from neonates and adults are comparable. Here we have directly compared neonatal and adult CD4+ RTEs for the first time, using a transgeni...

After transfer to adoptive hosts, in vitro-generated CD4 effectors can become long-lived memory cells, but the factors regulating this transition are unknown. We find that low doses of interleukin (IL) 7 enhance survival of effectors in vitro without driving their division. When in vitro-generated effectors are transferred to normal intact adoptive hosts, they survive and rapidly become small r...