Hypoxie Chinese hamster V-79 cells were examined for light-microscope morphology, progression through the cell cycle, chromosomal aberrations, and viability, after incubation with the 2-nitroimidazole, misonidazole [1-(2nitro-1-imidazolyl)-3 methoxy-2-propanol]. Cytological ex amination of cells up to 42 hr after incubation with the drug at 37°indicated that increasing contact time and increas...

 Abstract Background: To produce a reliable probe suitable for aneuploidy detection of chromosome 13 on uncultured lymphocytes and amniocytes by fluorescence in situ hybridization (FISH), we used a contig of three overlapping cosmids mapped to 13q12.3. Methods: The cosmid DNA carrying the expected sequences of human chromosome 13 was isolated from host cells and labelled with biotin-11-dUTP. Th...

1. Quantitative cytophotometric analysis of the interphase cells of a rapidly proliferating differentiated tissue such as liver of new born rat, indicates that these cells can be separated into two groups on the basis of their staining characteristics after methanol fixation. 2. These groups are thought to correspond to two stages of interphase. The first, called "autosynthetic interphase," com...

The experiments described in this paper have been designed to understand how particular dopants in alumina (Ca, Mg, Si, and Y) affect microstructural development through the energetics of their associated precipitates. Specifically, the role of the interphase boundary energy and precipitation activation energy are considered to be in competition with grain boundary complexion (disorder) transit...

Cenp-F (also named mitosin) is a 350-kDa human kinetochore protein important for the mitotic progression. It is also a nuclear matrix protein in interphase cells. Here, we showed that overexpression of N-terminal deletion mutants of Cenp-F containing the C-terminal 112 residues induced chromatin condensation into numerous aggregates of varying sizes in interphase nucleus, colocalizing with the ...

Fluorescent in situ hybridization (FISH) with a 21q11-specific probe (CB21c1) consisting of three non-overlapping cosmids has been applied to interphase amniocytes of pregnancies at increased risk for fetal aneuploidy (N = 78) and to interphase lymphocytes, cultured and uncultured, of patients referred for Down syndrome (N = 19 and 28, respectively). In the uncultured amniocytes, six chromosome...

In the current model of chromatin organization in the interphase cell nucleus, chromosomes are organized into territories. Although constrained diffusion of chromatin in interphase cells has been confirmed in all cell types examined, little is known about chromatin dynamics in plant interphase cells. In this work, we measured for the first time interphase chromatin dynamics in plants using the ...

The interphase transfer of turbulent kinetic energy TKE is an important term that affects the evolution of TKE in fluid and particle phases in particle-laden turbulent flow. This work shows that the interphase TKE transfer terms must obey a mathematical constraint, which in the limiting case of statistically homogeneous flow with zero mean velocity in both phases, requires these terms be equal ...