Contamination of seafood with salmonellae is a major public health concern. Detection of Salmonella by standard culture methods is time consuming. In this study, an enrichment culture step prior to polymerase chain reaction (PCR) was applied to detect 284 bp fragment of Salmonella invA in comparison with the conventional culture method in 100 shrimp samples collected from four different shrimp ...

Guano samples from 412 Brazilian bats were screened with real-time PCR for the virulence genes (eae, est, elt, stx1, stx2, ehxA, invA, bfpA, aggR) representing five intestinal pathotypes of Escherichia coli. From 82 pooled samples, 22% contained Escherichia coli DNA, and eae, est, bfpA, aggR were detected.

The objective of this study was to describe the shedding pattern of Salmonella in finishing pigs, as well to quantify the Salmonella load. A longitudinal study was conducted in 12 cohorts of pigs in a multi-site farrow-to-finish production system. At the beginning of each cohort, 50 pigs (10 ± 2 weeks old) were randomly selected and individually identified. Individual pig fecal samples were col...

The aims of this study were to isolation of Salmonella from poultry farms of Shiraz province of Iran and determination of their susceptibility against common antibiotics. One hundred and ninety two samples were harvested from intestine and liver of chickens and were aseptically cultured in enrichment and selective media. Out of 192 samples, 30 Salmonella were isolated. Four different serogroups...

Etiological diagnosis of diarrheal diseases may be complicated by their multi-factorial nature. In addition, Escherichia coli strains present in the gut can occasionally harbor virulence genes (VGs) without causing disease, which complicates the assessment of their clinical significance in particular. The aim of this study was to detect and quantify nine VGs (stx1, stx2, eae, aggR, ehxA, invA, ...

We have developed a colorimetric biosensor using a dual platform of gold nanoparticles and graphene oxide sheets for the detection of Salmonella enterica. The presence of the invA gene in S. enterica causes a change in color of the biosensor from its original pinkish-red to a light purplish solution. This occurs through the aggregation of the primary gold nanoparticles-conjugated DNA probe onto...

In this study, the microbiological quality of roof-harvested rainwater was assessed by monitoring the concentrations of Escherichia coli, enterococci, Clostridium perfringens, and Bacteroides spp. in rainwater obtained from tanks in Southeast Queensland, Australia. Samples were also tested using real-time PCR (with SYBR Green I dye) for the presence of potential pathogenic microorganisms. Of th...

background: salmonella typhimurium is one of the most important species of salmonella that is intracellular parasite and attacks host mucus membrane. these bacteria can cause gastroenteritis, and their main transmission route is water, poultry, meat, egg, and raw food. the aim of this study was to detect three virulence genes associated with s. typhimurium named inva, stm4497, and flic183 genes...