PURPOSE The well-known translocations identified in MALT lymphomas include t(11;18)/API2-MALT1, t(1;14)/IGH-BCL10, and t(14;18)/IGH-MALT1. Molecular investigations have suggested that these three disparate translocations affect a common pathway, resulting in the constitutive activation of nuclear factor-kappaB. However, the vast majority of MALT lymphomas are negative for any of the above-menti...

Adult T-cell leukemia (ATL) is neoplasm of the mature helper T lymphocytes and human T-cell lymphotropic virus type-I (HTLV-I) has been shown to be causative virus of ATL. Because HTLV-I integrates its provirus randomly into host chromosomal DNA, monoclonal integration of HTLV-I provirus indicates the clonal proliferation of HTLV-I-infected cells. Therefore, demonstration of clonality of HTLV-I...

in the linear system ax = b the points x are sometimes constrained to lie in a given subspace s of column space of a. drazin inverse for any singular or nonsingular matrix, exist and is unique. in this paper, the singular consistent or inconsistent constrained linear systems are introduced and the effect of drazin inverse in solving such systems is investigated. constrained linear system arise ...

background chronic hepatitis b (chb) is accompanied by inflammation of liver because of infection with hepatitis b virus (hbv). previous studies revealed an inverse association between vitamin d and hbv dna levels. objectives the current study aimed to investigate the levels of 25 (oh) d3 (the steady form of vitamin d), mir-378 and hbv dna in the patients with chb. patients and methods one hund...

In the era of the Human Genome Project, quantitation of gene expression by tumor/host cells is of paramount importance to investigate gene patterns responsible for cancer development, progression and response/resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstr...

T-vectors have been widely used in molecular cloning [1–3]. Compared with common vectors, molecular cloning with T-vectors escapes the restriction endonuclease digestion process, decreases the time of DNA purification and thus is time-saving and cost-effective. There are two ways to produce T-vectors: (i) cutting the target vector with EcoRV and then adding a thymine residue to the 3′-blunt end...

In the last several years, the use of double-stranded DNA templates together with thermostable-polymerase PCR has essentially replaced the use single-stranded DNA templates using the thermolabile polymerase for in vitro mutagenesis. Numerous PCR methods are now available, such as overlap-extension PCR, megaprimer PCR, and inverse PCR. All these PCR methods are reliable, effective, and convenien...