Values for serum albumin obtained by the methyl orange and 2-(4’-hydroxyazobenzene) benzoic acid (HABA) dye-absorption methods have been compared with values obtained by Spinco Procedure B paper-electrophoresis system. A good correlation of albumin values has been found by the three methods in serums with albumin/globulin ratios ranging from 0.19 to 3.00. Modifications of the dye-absorption pro...

Preliminary studies by Beers et a2. (1) have shown that the zinc salt of acridine orange can either inhibit or accelerate the rate of polymerization of polynucleotides by polynucleotide phosphorylase of Micrococcus lysodeileticus, depending upon the experimental conditions. We have now confirmed these findings with the zinc-free dye. Some evidence for a reversal of inhibition with degraded ribo...

(a) by competing for hydrogen bonding sites in the tissue or on the dye particle, it may inhibit staining in which the dye-substrate link is a hydrogen bond; (6) because it is a dipole, urea may have some affinity for charged sites in the tissue, and thus tend to reduce electrostatic staining; (c) urea may in some cases decrease staining by increasing the solubility of the dye; (d) by inhibitin...

Near-field scanning optical microscopy (NSOM) and atomic force microscopy (AFM) have been employed to spatially resolve the complex nanoscale morphologies, spectroscopy, and energy-transfer efficiencies of self-assembled multilayered structures composed of alternating layers of R-zirconium phosphate [R-Zr(HPO4)2] (ZrP) and dye-labeled poly(allylamine hydrochloride) (dye-PAH) (where dye ) Fluore...

Here, we demonstrate an anion controlled molecular gate based on synthetic ion channels modified with polyethyleneimine. For single conical nanochannels, addition of ATP leads to significant decrease in the rectified ion flux, representing the closure of the ionic gate. Complementary experiments performed with nanoporous membranes show that the flux of charged dye (NDS(2-)) through a cylindrica...

Autonomous sensing of metal ion contamination in remote environments with high reproducibility and sensitivity could unlock many new applications, but involves trade-offs between compactness, sensitivity, and power provisioning. In prior demonstrations of semi-autonomous sensors, the power source (e.g. a solar cell) was an additional component. Here, we demonstrate a concept, wherein a dye-sens...

Herein we report the molecular structures and electronic properties of ionic, hydrophobic, half-sandwich complexes of the formula [η(5)-Cp*Rh(L)(MeOH)] (1) and [η(6)-CyRu(L)(H2O)] (2), where L is azo-dye compound of (p-(2-hydroxy-1-naphthylazo)benzenesulfonic acid sodium salt). Both these complexes have been investigated electrochemically and found to display good electronic properties for use ...