An automated, qualitative screening HPLC method for the identification of basic compounds in urine has been established. A 1-ml volume of urine was extracted by on-line extraction and separated on two coupled strong cation-exchange columns under isocratic conditions. The use of the photodiode-array detector (DAD, = 190370 nm) gave access to a library of > 2600 toxicologically relevant compounds...

A method is described for the rapid purification of radioactively labeled deoxyribonucleoside triphosphates from their spontaneously emerging hydrolysis products deoxyribonucleoside diphosphate, deoxyribonucleoside monophosphate, and deoxyribonucleoside. The separations which are finished within 3 min or less are carried out on a 0.1 X 5 cm column filled with LiChrosorb-NH2, using isocratic elu...

Six compounds were analyzed through isocratic elution to demonstrate the effect of several instrument, method, and sample variables on column performance. A very efficient Agilent InfinityLab Poroshell 120 EC-C18, 2.1 × 50 mm, 1.9 μm column capable of generating nearly 14,000 plates was used in this work. The impact on column performance of system capillaries, detector flow cells, data collecti...

This paper describes potential applications of computer-assisted chemometrics in method development in liquid chromatography. These include modeling of retention (isocratic, gradient, molecular modeling, artificial neural networks), assessment of separation (peak capacity), single and multiple objective optimization approach, advanced optimization algorithms (genetic algorithms, simulated annea...

This work focuses on development of ad hoc methodology for monitoring of sodium, ammonium, potassium, magnesium and calcium ions in rivers Ilova and Kutinica (southeastern part of Croatia) since often their composition significantly differs from the average (concentration of components and/or matrix). Therefore fast and reliable method development has to be performed in order to meet criteria o...

Phenolic acids in commercially important sweet potato cultivars grown in the United States were analyzed using reversed-phase high-performance liquid chromatography (HPLC). Caffeic acid, chlorogenic acid, 4,5-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, and 3,4-di-O-caffeoylquinic acid were well separated with an isocratic elution in less than 25 min compared to about 120 min for ana...

INTRODUCTION Resperidone chemically 4-[2-[4-(6-fluorobenzo [d] isoxazol-3-yl)-1-piper idyl] ethyl] -3methyl-2, 6diazabicyclo [4.4.0] deca-1, 3-dien-5-one. This drug belongs to a class of Anti-psychotic known as atypical neuroleptics. It is a strong dopamine antagonist. It has high affinity for D2 dopaminergic receptors. It has actions at several 5-HT (serotonin) receptor subtypes. The latter ac...