To the Editor: The last step in electrophoretic procedures for creatine kinase (CK; EC 2.7.3.2) isoenzyme separation on cellulose acetate is to incubate the plate with a reagent that will develop either fluorescence or color for each isoenzyme. In our laboratory we routinely use fluorescence to detect the isoenzymes. The reagent used must be highly sensitive, so that low proportions of the isoe...

Serum samples from patients receiving intravenous streptokinase were examined for evidence of interaction in vivo between streptokinase and lactate dehydrogenase (EC 1.1.1.27; LD). We found that this treatment produced a band of LD activity that remained at the electrophoretic origin of LD isoenzyme analysis. Treatment with tissue plasminogen activator produced no such band. The streptokinase-L...

I describe a simple, rapid anion-exchange column chromatographic technique for separating the creatine kinase (CK) isoenzymes in human serum and tissue. Extracts of CK-rich tissues (skeletal muscle, cardiac muscle, and brain) were used to determine optimum conditions for separating CK isoenzymes MM, MB, and BB. Samples, layered on mini-columns (0.5 X 6.0 cm) of DEAE-Sephadex A-50, were eluted s...

Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. In the presence of 1.6 mM-NAD+, isoenzyme LDH-X does not bind to AMP-Sepharose, whereas the other lactate dehydrogenase isoenzymes do. This is the crucial point in the isolation of isoenzyme LDH-X from the othe...

Fig. 1. Electropherogram stained for alkaline phosphatase isoenzymes A. albumin with added biiWubin; B, billrubin solution In saline; C, patient’s serum with artifactual band in addition to placental and lIver isoenzyme bands (poody resolved); D. intestinal and placental isoenzyme control; E, patient’s serum with liver Isoenzyme band; F, Intestinal and live’ lsoenzyme control; 0, patient’s seru...

Serum and tumor tissue of a patient with neuroblastoma contained an abnormal isoenzyme of lactate dehydrogenase (LDH; EC 1.1.1.27), which, on agarose gel electrophoresis, migrated between LDH-2 and LDH-3 with a mobility the same as that of the extra LDH isoenzyme found in normal human erythrocytes. On surgical removal of the tumor, the high total LDH activity (775 U/L) in the serum of the patie...

Previously we have shown that aldolase (ALD; EC 4.1.2.13) is present in cardiomyocyte nuclei. Now, we focused our attention on ALD localization in smooth muscle cells. Immunocytochemical methods were used to study the subcellular localization of ALD. Aldolase was localized in the cytoplasm as well as in the nuclei. Within the nuclei ALD was located in the heterochromatin region. Native polyacry...

We studied possible correlations between anatomopathological and clinical features and the values for total creatine kinase (CK; EC 2.7.3.2) and its isoenzymes, including the proportion of CK-MB, in a population displaying several neuromuscular pathologies. Although we observed no specific isoenzyme pattern associated with the different myopathies, we found isoenzyme analysis useful in studying...

Human adenosine deaminase (ADA; EC 3.5.4.4) consists of three isoenzymes: ADA1, ADA1+CP, and ADA2. We developed an electrophoretic technique to distinguish between these three isoenzymes. The isoenzyme pattern was studied in tissue and cell homogenates, as well as in serum from normal subjects and from patients with increased serum ADA who had either hepatitis, infectious mononucleosis, tubercu...

To evaluate the combined effects of cardiac overload imposed by hypertension and by chronic exercise, male and female rats were made hypertensive by unilateral renal artery stenoses and made to exercise in an 8-10-wk swimming program. Sedentary normotensive animals, sedentary hypertensive animals and normotensive animals exposed to the swimming program were also studied. Hypertension was associ...