A novel stimulatory monoclonal antibody (Mab) termed Mab.F11 induces granular secretion and subsequent aggregation of human platelets. Mab.F11 recognizes a unique 32 and 35 kDa protein duplex on the platelet membrane surface, called the F11 receptor; binding of Mab.F11 to its receptor results in increased intracellular phosphorylation of P47, the known protein kinase C (PKC) substrate pleckstri...

OBJECTIVE The present study was carried out to determine the effect of cadmium exposure on Urinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A and B in workers exposed at cadmium plating. METHODS 50 subjects using cadmium during cadmium plating formed the study group. An equal number of age-sex matched subjects working in administrative section formed the control group. Urinary cadm...

In this work, the characterization of endoprotease (EP) isoenzymes in peroxisomes is reported for the first time in cell organelles purified from pea leaves (Pisum sativum L.). A comparative analysis of the endo-proteolytic activity in peroxisomes purified from young (15-day-old) and senescent (50-day-old) leaves was carried out. Peroxisomes purified from senescent leaves showed a much higher e...

At 2 weeks of age, 11 isoenzymes were expressed and similar banding patterns on vertical polyacrylamide gel electrophoresis (PAGE), stained by alpha- or beta-naphthyl acetate as a substrate, were obtained for tissues or fluids from the proximal and the distal parts of the mouse epididymis. After this period, the emergence of new bands or the disappearance of certain others led to a regional dif...

A simple, sensitive staining procedure for the localization of leucine aminopeptidase isoenzymes after electrophoretic separation on cellulose acetate membranes is described. Separation of leucine aminopeptidase isoenzymes, using cellulose acetate membranes, does not appear to be a rewarding diagnostic procedure in the investigation of hepatobiliary or pancreatic disorders.

The isoenzymes ADA1 and ADA2 of the enzyme adenosine deaminase (ADA 3.5.4.4) deaminate mainly two nucleosides: adenosine and 2'deoxyadenosine, producing inosine and 2'deoxyinosine. Adenosine and 2'deoxyadenosine are molecules with many effects on human cells [1–4]. Thus, the homeostasis of these substances and the activity of the isoenzymes ADA1 and ADA2 in human cells are of extreme importance.

A simple and specific detection method for multiple forms of cystine aminopeptidase (oxytocinase-isoenzymes) in pregnancy serum is described using S-benzyl-L-nitroanilide as substrate. The relative distribution of both cystine aminopeptidase isoenzymes at different stages of pregnancy is given.

The isoenzymes of alkaline phosphatase (AP) in the serum of 30 normal subjects and 90 patients with common hepatobiliary disorders are distinguished using electrophoresis on cellulose acetate. Physicochemical profiles of these mixtures of serum isoenzymes are presented using thermostability and organspecific inhibitors (urea, L-phenylalanine, and L-homoarginine). The findings are reviewed and t...

The structural relationships among human alkaline phosphatase isoenzymes from placenta, bone, kidney, liver and intestine were investigated by using three criteria. 1. Immunochemical characterization by using monospecific antisera prepared against either the placental isoenzyme or the liver isoenzyme distinguishes two antigenic groups: bone, kidney and liver isoenzymes cross-react with anti-(li...

Tyrosinase, which catalyzes the formation of melanin from tyrosine (Eq. 1) has only recently been purified to homogeneity from mammalian sources (1, 2). This paper reports a rapid and improved method of purification of one of the several tyrosinase isoenzymes from hamster melanoma and the determination of its amino acid composition. The molecular weights of three of the isoenzymes have been det...