In this study Artemisia amygdalina a critically endangered medicinal plant endemic to Kashmir Himalayas was grown under in vitro conditions. ISSR markers were used to study the somaclonal variation at the genetic level. Two ISSR-primers (CAC)3 GC and (ACTG)5 were used, two additional DNA bands (0.15kb and 0.2kb) were observed respectively in tissue cultured Artemisia amygdalina. Direct regenera...

The work involved a molecular biological technique (ISSR-PCR) using ISSR-primer S-2, with structure (AGC) 6G. Changes in the gastric mucosa chronic duodenal ulcer disease against background of severe atrophic gastritis have been analyzed. Noteworthy is fact that there strong correlation between degree dysplasia epithelium and mitotic index, Pearson's coefficient rxy was 0.853, respectively. A v...

micropropagated plantlets derived from three different grape rootstock genotypes namely, dogridge (vitis champini), so4 (v. berlandieri×v. rupestris) and ari-h-144 (v. vinifera×v. labrusca) were subjected to randomly amplified polymorphic dna (rapd) and inter simple sequence repeats (issr) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vi...

Date palm (Phoenix dactylifera L.) is a monocot, dioecious and high longevity tropical plant greatly considered due to its high economic importance in Middle East. This investigation was carried out to detect genetic relations among 34 cultivars of Iranian date palm, both males and females, using ISSR markers. Accordingly, total DNA was extracted using CTAB method and was subjected to PCR ampli...

In this dataset, we present 15 Simple Sequence Repeat (SSR) markers with the motifs (AC)n, (GA)n, and (AC)n(AG)n using a ISSR-Suppression-PCR technique in order to discriminate Garcinia mangostana from diverse geographical origins in Peninsular Malaysia. A few loci showed differences between 3 and 6 bp in allele size, indicating that there are some polymorphisms between individuals correlating ...

the kermani sheep is an important meat producing an animal in iran and its economic efficiency is mainly dependent on its growth and reproduction ability. inter-simple sequence repeat (issr) is the genome region between microsatellite loci. the aim of this study was to evaluate the association of two different issr markers with predicted breeding values (pbv) of body weight trait in kermani she...

the flowering and fructification period of the chilean strawberry (fragaria chiloensis (l.) duch.) is restricted to approximately 2 mo, which seriously limits the commercial development of the species. the objective of the current investigation was to identify inter simple sequence repeat (issr) polymorphisms associated with flowering duration in accessions of f. chiloensis. the flowering durat...

Sequence-related amplified polymorphism (SRAP), amplified fragment length polymorphism (AFLP), and inter-simple sequence repeat (ISSR) markers were used to estimate the genetic diversity and relationships among Eucommia ulmoides cultivars in China. A total of 240, 192, and 150 DNA fragments were detected by 10 SRAP primer combinations, 10 AFLP primer combinations, and 10 ISSR primers, among whi...

Five Hesperocyparis (Cupressus) abramsiana groves were analyzed by Inter-Simple Sequence Repeats (ISSRs). ISSRs analyses revealed geographical differentiation among the groves (= Silba's subspecies) with the Butano Ridge grove being the most distinct in both ISSRs and terpenoids. Combined ISSR and terpenoid data support the recognition of Hesperocyparis. abramsiana var. abramsiana and Hesperocy...

Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) and inter simple sequence repeats (ISSRs) markers were performed to detect the genetic diversity among 6 new rice lines and 4 cultivars with different responses to drought tolerance and establish specific DNA markers associated with drought tolerance. Among 16 RAPD primers tested, only 5 produced bands polymorphic between lin...