l h e gene encoding thioredoxin in the facultative heterotrophic cyanobacterium Synechocysfis sp. PCC 6803 (trxA) has been cloned by heterologous hybridization using the corresponding gene frxM from the cyanobacterium Anacysfis nidulans as a probe. l h e deduced amino acid sequence of frxA predicts a protein of relative molecular weight of 11,750 and has strong identity with its cyanobacterial ...

Cumin (Cuminum cyminum L.) is an annual plant belonging to the Apiaceae family is one of the oldest and economically most important medicinal spices. This study was conducted to determination of threshold kanamycin concentration in cumin for the purpose of gene transfer in a completely randomized design with three replications. Percentage of regenerated plants was recorded to study kanamycin ef...

Previous studies have shown that pretreatment of E. coli with low doses of kanamycin have generated an increase in the resistance to high doses of kanamycin as well as induced cross resistance to a broad range of unrelated antibiotics, and that multiantibiotic-resistant E. coli had lower levels of OmpF porin. In this study, the role of OmpC and OmpF porins in the development of cross resistance...

It was recently reported that the dosage of kanamycin formerly recommended for neonates of 7.5 mg/kg every 12 h did not produce therapeutic serum concentrations of 15 to 25 mug/ml, but a larger dosage of 10 mg/kg every 12 h was required. Reevaluation of the pharmacokinetics of kanamycin in infants and children was therefore undertaken. Sixteen time-concentration curves after a dose of 5 mg/kg w...

SUMMARY The impact of a Pseudomonas fluorescens strain, genetically modified for kanamycin resistance and lactose utilisation (the GMM), could be enhanced by soil amendment with lactose and kanamycin. Lactose addition decreased the shoot to root ratio of pea, and both soil amendments increased the populations of total culturable bacteria and the inoculated GMM. Only kanamycin perturbed the bact...

background and objectives: during past decades hansenula polymorpha has attracted global attention for the expression of recombinant proteins due to its high growth rate, minimal nutritional porequirements and use of methanol as a low cost inducer. materials and methods: the corresponding nucleotide sequences for the expression of heterologous genes in hansenula poylmorpha were extracted and as...

A 5 day topical kanamycin treatment of the dental gingival surfaces in institutionalized mentally retarded subjects significantly reduced the plaque mass on the tooth surfaces compared to a placeho treatment. This reduction occurred in the absence of any mechanical hygiene procedures and lasted for 4 or more weeks, which was longer than the kanamycin could have been expected to be present in th...

Kanamycin is an aminoglycoside antibiotic produced by Streptomyces kanamyceticus1). Although it possesses broad range antibacterial activity, bacteria resistant to this antibiotic readily appear, and therefore its clinical use has been restricted to tuberculosis. Resistant bacteria inactivate kanamycin by modifying its specific aminoor hydroxygroup using acetyltransferase, phosphotransferase an...

Monoclonal antibody against kanamycin was prepared, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. The monoclonal antibody produced was identified to be IgG1, which has a kappa light chain. No cross-reactivity of the antibody was detected with other aminoglycosides, indicating that the monoclonal anti...