The vav gene is expressed in all hematopoietic but few other cell types. To explore its unusual compartment-wide regulation, we cloned the murine gene, sequenced its promoter region, identified DNase I hypersensitive (HS) sites in the chromatin, and tested their promoter activity with a beta-galactosidase (beta-gal) reporter gene in cell lines and transgenic mice. Whereas fibroblasts had no HS ...

objective(s) the cytotoxic effects of crude ethanol extracts of some previously tested iranian conifers  on tumor cell lines have motivated us to screen different parts of two subspecies in these genus. materials and methods terminal branchlets and berries of juniperus excelsa subsp. excelsa and j. excelsa subsp. polycarpos were collected, dried and extracted with ethanol/h2o (80/20 v/v) via pe...

The B subunit is one of two nucleotide-binding polypeptides found in all members of the vacuolar class of H(+)-translocating ATPases. We have isolated aDNA clone encoding the bovine brain B (58 kDa) subunit and have deduced its amino acid sequence. The bovine brain amino acid sequence is 99% identical to a partial cDNA reported from human brain. Northern blot analysis of RNA isolated from bovin...

To identify critical amino acid residues recognized by alloreactive cytolytic T lymphocytes (CTL) generated between H-2Kb and H-2Kbm1, we have derived a series of cloned L-cell lines expressing the following mutant H-2Kb class I genes. Cell line L-KbTyr-Tyr expresses a mutant gene in which positions 155-156 of the Kb molecule have been changed from Arg-Leu to Tyr-Tyr, leaving position 152 uncha...

We report thermal, X-ray diffraction (XRD) and cytotoxicity studies of complexes of fluconazole (FCZ) with Cu (II), Fe(II), Cd(II), Co(II), Ni(II), and Mn(II). From XRD measurements, FCZ and its metal complexes were identified as polycrystalline. Marked differences in the X-ray patterns of drug and its metal complexes revealed that the complexes are indeed different compounds and not just the m...

BACKGROUND The biological effects of cytokines are coming to be understood. The therapeutic effects of interleukin (IL) 2, IL-12, and interferon gamma (IFN-gamma) in cancer treatment have been reported, but there are problems when these cytokines are systemically used as therapeutic agents. OBJECTIVE To examine the efficacy of IL-2 and IL-12 gene-transfected tumor cell vaccines for head and n...

objective: nucleostemin (ns) is a recently identified gene that is expressed mainly in the nucleoli of neuronal, embryonic and mesenchymal stem cells which plays a role in their self renewal. over expression of this gene has been reported in several cancer cell lines tumoral tissues including gastric, hepatic and prostate cancer. in this study, we investigated the effects of suppression of this...